[Abstract Title]. - Society for Neuroscience

common dementia among older people and is the 4th cause of death in developed countries.
Many studies are focused on Tau protein which is the principal component of neurofibrillary
tangles (NFT). Tau localization is changed during the pathology development. However it had
not been reported the analysis of Tau membrane localization and its importance in the
pathological Tau precipitation. The purpose of this job was to develop a cellular model to
analyze the possible role of the miss-localization of truncated Tau in AD neurodegeneration. We
used 2 gene constructs: 1) soluble truncated Tau (151-391 aa) and 2) a chimerical form with a
membrane anchored signal (IFNγR1-151-391). Through retroviral system and transfection
analysis, we expressed the constructions in neuronal culture. We noticed the presence of β-sheet
structures when we induce differentiation from neural stem cells to neuronal culture in the
transduced cell that expressed the Tau anchored to membrane. These data suggests the
participation of neuronal membrane as nucleation center to abnormal aggregation of Tau and
sustain the importance of truncation as a post-translation modification to the development of AD.
Disclosures: M.A. De Anda-Hernandez, None; K.I. Lira-De Leon, None; P. Figueroa-
Corona, None; M.A. Meraz-Rios, None; V. Campos-Peña, None.
Poster
245. Tau and Alzheimer's disease
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 245.21/P3
Topic: C.01.j. Cognitive function
Support: NIH grant AG 05146
Intramural Research Program of the NIA, NIH.
Burroughs Wellcome Fund for Translational Research
Title: No quantitative differences in 1-42β-amyloid and hyperphosphorylated-tau tangles loads
in the brains of subjects with asymptomatic AD and mild cognitive impairment (MCI). The
BLSA study
Authors: *D. IACONO 1 , R. O' BRIEN 2 , S. M. RESNIK 3 , A. ZONDERMAN 3 , O.
PLETNIKOVA 1 , G. RUDOW 1 , B. CRAIN 1 , J. C. TRONCOSO 1 ;
1 Dept Pathol, Div. Neuropathol, 2 Dept of Neurol., Johns Hopkins Univ., Baltimore, MD;
3 Personality and Cognition Lab., Natl. Inst. of Aging, Baltimore, MD