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[Abstract Title]. - Society for Neuroscience

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<strong>Title</strong>: Extracellular levels of GABA and glutamate in the ventral pallidum of the alcohol<br />

preferring AA and alcohol avoiding ANA rats after administration of ethanol<br />

Authors: *K. KIIANMAA, N. RAIVIO, H. NURMI, H. KEMPPAINEN;<br />

Dept Mental Hlth. & Alcohol Res., Natl. Publ. Hlth. Inst., 00251 Helsinki, Finland<br />

<strong>Abstract</strong>: Ventral pallidum serves as a major terminus <strong>for</strong> accumbal efferent projections, which<br />

are GABAergic. These projections have been viewed as the final common link in the brain<br />

reward circuitry. The purpose of the present study was to clarify the role of the ventral pallidum<br />

in the regulation of ethanol consumption by studying the extracellular levels of GABA and<br />

glutamate in the ventral pallidum after acute administration of ethanol. Studies were per<strong>for</strong>med<br />

with in vivo microdialysis in drug naive alcohol preferring AA (Alko Alcohol) and alcohol<br />

avoiding ANA (Alko Non-Alcohol) rats. Microdialysis probes were implanted in the ventral<br />

pallidum and artificial cerebrospinal fluid was pumped in awake and freely moving animals at a<br />

rate of 1,5 κl/min. Dialysate was collected at 20-minute intervals. Following a baseline period<br />

(120 minutes, 6 samples), the rats were injected with physiological saline or ethanol (1 or 2 g/kg,<br />

IP). The collection of the samples was continued <strong>for</strong> 240 min. The concentrations of GABA and<br />

glutamate were determined with a HPLC system using fluorescent detection. Ethanol<br />

significantly decreased the extracellular levels of GABA. The ANA rats were more sensitive to<br />

this effect of ethanol than AA rats. Ethanol didn‟t have any effect on the concentrations of<br />

glutamate in either rat line. The suppressive effect of ethanol on the extracellular levels of<br />

GABA in ventral pallidum suggest a role <strong>for</strong> pallidal GABAergic transmission in the control of<br />

ethanol consumption.<br />

Disclosures: K. Kiianmaa , None; N. Raivio, None; H. Nurmi, None; H. Kemppainen, None.<br />

Poster<br />

257. Alcohol Intake and Preference<br />

Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm<br />

Program#/Poster#: 257.15/Z32<br />

Topic: C.16.a. Alcohol<br />

Support: SC-INBRE<br />

NIAAA/NIH Intramural Research Program<br />

<strong>Title</strong>: The effects of chronic ethanol intake on brain glucose utilization in mice lacking the<br />

cannabinoid 1 receptor

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