[Abstract Title]. - Society for Neuroscience

assays further confirm the ability of PPAR gamma to bind to predicted PPREs. These results
implicate PGC-1 alpha and PPAR gamma in a coordinated response during cellular oxidative
stress. Altogether this project will determine the feasibility of PGC-1 alpha overexpression as a
protective strategy against ROS-induced neuronal cell death, and provide a better understanding
of the mechanisms by which PGC-1 alpha acts in neuronal cells.
Disclosures: J.M. Silvaggi, None; C. Chung, None; A. Sheets, None; J. Clark, None; D.K.
Simon, None; O. Isacson, None; B.M. Spiegelman, None.
Poster
253. Parkinson's Disease Models: Neuroprotective Mechanisms I
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 253.11/X12
Topic: C.11.j. Neuroprotective mechanisms: Parkinson‟s disease models
Title: Effect of the C-terminal chain of tetanus toxin on the motor behaviors in hemiparkinonian
rats
Authors: *L. M. MARTINEZ MENDIETA 1 , B. VENEGAS 2 , A. PATRICIO 2 , I.
MARTÍNEZ 2 , J. AGUILERA 3 , I. LIMÓN 2 ;
1 Dept Pharmacy, Neuropharmacol, Benemerita Univ. Autonoma de Puebla, Puebla, Mexico;
2 Dept Pharmacy, Neuropharm., Benemérita Univ. Autónoma de Puebla, Puebla, Mexico;
3 Bioquímica i de Biologia Mol., Univ. Autónoma de Barcelona, Barcelona, Spain
Abstract: Currently treatments for PD alleviating symptoms of the disease so only restore the
motor function temporally but not stop the dopaminergic neurodegeration. Thus, it is essential to
develop new alternatives preventing ongoing cell death in this disorder. A successful therapeutic
use the glial derived neurotrophic factor (GDNF), a potent trophic factor that improves motor
behaviors, and protects tyrosine hydroxylase (TH) nigral neurons and enhance dopamine levels.
Unfortunately, all the neurotrophins doesn´t pass the blood-brain barriers and need repeated
administration for this reason is more difficult their use for PD. Recently, has been shown that
the C-terminus fragment of the tetanus toxin (Hc-TeTx) is transported retrogradly and had shown
neuroprotective effects, preventing neuronal death by apoptosis in cerebellar granule neurons in
cultures. Moreover the first results have shown the efficacy of Hc-TeTx on ameliorating
behavior deficits and improvement of dopaminergic system, were evaluated in turn behavior,
fine motor skills, asymmetry of both forelimbs in MPP + -lesioned rats. The aim of this study was
to evaluate the effects of Hc-TeTx in 6-OHDA or MPP + treated rats using motor behavioral tests
and striatal survival. Male Wistar rats (250-300g) were unilateral injected with 2 µM of Hc-TeTx
previous to 6-OHDA 2 µl (8 µg/µl) or MPP + 1µL (10µg/µL) in the nigrostriatal pathways or left