[Abstract Title]. - Society for Neuroscience

ut the biological role and tissue distribution of the peptides encoded by these genes is not well
studied. We examined the distribution of urocortin-1 (UCN-1) and UCN-3 producing neurons in
a model anuran species, the Korean fire-bellied toad Bombina orientalis. We used polyclonal
antisera raised in rabbits against an oligopeptide sequence from frog UCN-3 or an evolutionarily
conserved region in the C-terminus of rat UCN-1. Intensely stained UCN-3-immunoereactive (ir)
perikarya were found in the infundibular hypothalamus and preoptic area, while immunostained
fibers were found throughout the septal nuclei, in the bed nucleus of the stria terminalis,
amygdala, and throughout the hypothalamus. Interestingly, many UCN-3-ir fibers (probably
dendrites) penetrated the ependyma to contact cerebrospinal fluid. Isolated UCN-1-ir neurons
were observed in periventricular areas of the preoptic area and hypothalamus. Intensely-stained
UCN-1-ir neurons were observed in the vicinity of the motor nucleus of the oculomotor nerve, a
UCN-1 cell group that may be homologous to the well-described Edinger-Westphal (EW) UCN-
1 cell group first reported in mammals. Our data suggest intense innervation of forebrain limbic,
basal ganglia, and hypothalamus by UCN-3 neurons, providing strong support for a role of UCN-
3 in regulating autonomic and endocrine homeostasis. The finding of UCN-1 in putative
oculomotor regulatory areas of midbrain is consistent with the fact that the EW nucleus is the
principal site of UCN-1 in the mammalian brain.
Disclosures: N.B. Bergfeld , None; S. Bulin, None; N. Barawid, None; J.A. Carr, None.
Poster
283. Stress-Regulated Pathways II
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 283.18/PP14
Topic: E.06.f. Stress modulated pathways
Support: NIMH Grant MH 52619
Eli Lilly & Co.
Title: CBiPES, a potent group II metabotropic glutamate receptor allosteric potentiator, prevents
sodium lactate-induced panic-like response in panic-prone rats
Authors: *S. D. FITZ 1 , P. L. JOHNSON 1 , K. A. SVENSSON 2 , J. S. SCHKERYANTZ 2 , A.
SHEKHAR 1 ;
1 Psychiatry, Indiana Univ. Schl Med., Indianapolis, IN; 2 Eli Lilly & Co, Indianapolis, IN
Abstract: Adult male rats with chronic inhibition of GABA synthesis (with l-allyglycine, a
glutamic acid decarboxylase inhibitor) in the dorsomedial hypothalamus/perifornical area