[Abstract Title]. - Society for Neuroscience

ostral CC, while myelinated or demyelinated axons had respectively similar diameters within
each region. Neurofilament dephosphorylation, an indictor of disrupted axonal cytoskeletal
structure, was also more extensive in the caudal versus rostral CC. Our data in this cuprizone
model demonstrate that the early progression of pathology is more advanced in the caudal CC.
Furthermore, DTI axial diffusivity may be more sensitive to the disruption of neurofilament
structural integrity and less indicative of axonal atrophy. Supported by NIH NS39293, NMSS
RG3515 and DBSCIP F170TP.
Disclosures: J.E. Tobin, None; M. Xie, None; S. Song, None; T.Q. Le, None; D. McDaniel,
None; R.C. Armstrong, None.
Poster
251. Demyelinating Disorders: Animal Models and Human Studies I
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 251.14/W7
Topic: C.08.b. Animal models
Support: grant EU-NEST 1702
SNSF Grant PP00A-106714
Grant GM-28140
Grant SSMBS1140
Title: Phosphatidic acid mediates demyelination in Lpin1 mutant mice
Authors: K. NADRA 1 , A.-S. DE PREUX CHARLES 1 , J.-J. MÉDARD 1 , W. T. HENDRIKS 2 ,
G.-S. HAN 3 , S. GRÈS 4 , G. M. CARMAN 3 , J.-S. SAULNIER-BLACHE 4 , M. H. G.
VERHEIJEN 2 , *R. CHRAST 1 ;
1 Dept Med. Genet, Univ. Lausanne, Lausanne, Switzerland; 2 Dept. of Mol. and Cell. Neurobio.,
Vrije Univ., Amsterdam, Netherlands; 3 Dept. of Food Sci., Rutgers Univ., New Brunswick, NJ;
4 Dept. of Metabolism and Obesity, INSERM, U858/I2MR, Toulouse, France
Abstract: Lipids play crucial roles in many aspects of glial cell biology, affecting processes
ranging from myelin membrane biosynthesis to axo-glial interactions. In order to study the role
of lipid metabolism in myelinating glial cells, we specifically deleted in Schwann cells the Lpin1
gene, which encodes the Mg2+-dependent phosphatidate phosphatase (PAP1) enzyme necessary
for normal triacylglycerol biosynthesis. The affected animals developed pronounced peripheral