[Abstract Title]. - Society for Neuroscience

the vulnerability of the staging method to inter-rater bias has yet to be resolved. We tried to
establish an extension staging paradigm for AA in order to minimize this inter-rater bias.
TISSUE SOURCE: The brains from 217 consecutive general autopsy cases at a general geriatric
hospital since April 2005, as well as ten archival autopsy cases of AA with lobar hemorrhage
since January 1995, which were registered to the Brain Bank for Aging Research (BBAR) were
employed for this study. METHOD: The brains were fixed in formalin and embedded in paraffin.
Six-κm-thick serial sections were obtained from blocks of the frontal pole (F), hippocampus (H),
superior temporal gyrus (T1), interparietal sulcus (P), posterior cingulate gyrus (Ci), precuneus
(Pc), occipital pole (O), insular cortex (I), putamen (Pu), thalamus (Th), cerebellar hemisphere
(CH), midbrain (MB) and cervical cord (CC). The sections were evaluated with hematoxylin and
eosin, Kluever- Barrera and Congo red stainings, and immunohistochemically stained with antiamyloid
beta 11-28 and 1-40 antibodies. RESULT: Of the 217 cases overall, 174 cases
demonstrated AA and 7 among these showed small cerebral cortical hemorrhages caused by AA.
The incidence of AA in each anatomical location showed the following order of frequency: O,
137 cases; P, 128 cases; T1, 119 cases; F, 116 cases; Pc, 115 cases; Ci, 115 cases; CH, 81 cases;
I, 64 cases; H, 29 cases; CC, 29 case; MB, 19 cases; Pu, 13 cases and Th, 8 cases. The ten cases,
showing lobar hemorrhage, exhibited AA in the F, T1, P, O, I, CH and H. The hierarchy of AA
appearance was as follows: P/O> F/T1> CH> H> Pu. ApoE genotypes of the 17 cases showing
lobar or small hemorrhage were as follows: e2/e3, 4 cases; e3/e3, 10 cases; and e4/e4, two cases.
DISCUSSION and CONCLUSION: Our studies propose the following extension stage: Stage 0,
no AA; Stage A, AA only in P/O; Stage B, extending to F/T1; Stage C, spreading to CH; Stage
D, further involving H; and Stage E, reaching Pu. Those cases of lobar or small hemorrhages are
categorized into Stage D or E. Immuno- stainings with anti- Abeta antibody of occipical pole and
hippocampus, first, subsequently followed by that of cerebellum and putamen are sufficient for
this staging system. This extension stage fo AA shows a good correlation with the present
staging schemes for AA and is suitable to prepare morpholigical basis for amyloid imaging,
which causes attention to the deposition of amyloid beta to putamen. Our study also confirms an
association of apoE e2/e3 and e4/ e4 genotypes with AA.
Disclosures: H. Hatsuta, None; S. Murayama, None; Y. Saito, None; R. Sengoku, None; T.
Adachi, None; T. Arai, None; M. Sawabe, None; H. Mori, None.
Poster
243. Abeta Assembly and Deposition
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 243.3/J9
Topic: C.01.b. Abeta assembly and deposition
Support: SCOR 2P50HL60296