[Abstract Title]. - Society for Neuroscience

Title: Transient receptor potential A1 mediates acetaldehyde-evoked pain sensation
Authors: *S. HWANG, S. BANG, K. KIM, S. YOO;
Grad Sch. Med., Korea Univ., Seoul, Republic of Korea
Abstract: Six transient receptor potential (TRP) ion channels expressed in the sensory afferents
are playing an important role as body thermosensors and also peripheral pain detectors. It‟s
known that a number of natural compounds specifically activate those thermo sensitive TRP
channel and a well-known example is cinnamaldehyde for TRPA1. Here we show that human
and mouse TRPA1 is activated by acetaldehyde, an intermediate substance of ethanol
metabolism, in HEK293T cell heterologous expression system and culture of mouse trigeminal
neurons. Acetaldehyde failed to activate other temperature-sensitive TRP channels expressed in
sensory neurons. A TRPA1 antagonist camphor and a general TRP blocker ruthenium red
inhibited TRPA1 activation by acetaldehyde. Camphor and ruthenium red also suppressed acute
nociceptive behaviors induced by intradermal administration of acetaldehyde into the mouse
footpad. Intradermal coapplication of prostaglandin E2 with acetaldehyde greatly potentiated the
acetaldehyde-induced nociceptive responses and the effect was reversed by treatment of the
TRPA1 antagonist camphor. These results suggest that acetaldehyde causes nociception via
activation of TRPA1. Our data also may help elucidation of acetaldehyde-related pathological
symptoms such as hangover pain.
Disclosures: S. Hwang , None; S. Bang, None; K. Kim, None; S. Yoo, None.
Poster
265. Nociceptors II
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 265.9/EE10
Topic: D.08.a. Pain transduction molecules and channels
Support: KFO 100/1-2
Title: Modulation of TRPV1 activity during Opioid withdrawal
Authors: V. SPAHN, *M. SCHAEFER, C. ZOELLNER;
Anesthesiol and Intensive Care Med., Charité, Campus B. Franklin, Berlin, Germany
Abstract: Vanilloid receptor type 1 (TRPV1) is a ligand-gated ion channel expressed on sensory
nerves that responds to noxious heat, protons, and chemical stimuli such as capsaicin. TRPV1
plays a critical role in the development of tissue injury, inflammation or nerve lesions. Opioids