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[Abstract Title]. - Society for Neuroscience

[Abstract Title]. - Society for Neuroscience

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ehavioral sensitization. Behavioral sensitization may be a useful animal model of<br />

schizophrenia. The mechanism of METH induced behavioral sensitization is still unknown, but<br />

several evidences suggested dopaminergic system is underlying its fundamental roles. In the<br />

previous study, we obtained preliminary data indicating that PDLIM5 may play a role in the<br />

pathogenesis of schizophrenia through it‟s involvement in the dopaminergic signaling cascade.<br />

To investigate the involvement of Pdlim5 in an animal model of schizophrenia, we generated<br />

Pdlim5 knockout mice using Pdlim5 genetrap embryonic stem (ES) cells. An ES cell line<br />

containing genetrap vector was inserted in intron 8 of Pdlim5. Mice lacking Pdlim5 die during<br />

early developmental stage. Pdlim5 hetero knockout and wild type littermate male mice were<br />

treated with chronic METH (3mg/kg, once a day, intraperitoneal (i.p.)) <strong>for</strong> 10 days and<br />

developed behavioral sensitization to METH challenge injection (1mg/kg, i.p.) on withdrawal<br />

day 14. However, the Pdlim5 hetero knockout mice have attenuated the maintenance of<br />

behavioral sensitization of METH compared with wild type mice (p

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