[Abstract Title]. - Society for Neuroscience

vitro assay for factors that modulate progenitor cell production, since the optic nerve is not a
neurogenic area per se.
Image analysis of fluorescence intensity with Image Pro software showed that the optic nerve
cells that were co-cultured with microglia isolated from LPS activated brains show an increase in
the percentage of TUJ-1 positive cells (P=0.01). Monocytes have no effect on cell proliferation
and differentiation when co-cultured with optic nerves from neonatal rats.
To investigate the possible source of optic nerve generated neurons, A2B5 positive cells were
panned and the number of TUJ-1 positive cells with the presence of microglia derived from LPS
and control brains was counted. Interestingly, there is no difference in the number of TUJ-1 +
cells derived from A2B5+cells that were co-cultured with activated or inactivated microglia.
Disclosures: A.M. Nikolakopoulou, None; B.D. Trapp, None; A. Zaremba, None; R.H.
Miller, None.
Poster
229. Neuronal and Glial Proliferation III
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 229.23/A23
Topic: A.02.a. Proliferation
Support: NIH Grant R01-MH69686-05
Title: The long-term effects of selective brain lesions on adult neurogenesis in the rhesus
monkey brain
Authors: *D. KIM, L. B. NGWENYA, L. A. WELKE, R. J. KILLIANY, M. B. MOSS, D. L.
ROSENE;
Anat. and Neurobio., BUSM, Boston, MA
Abstract: The effects of brain damage on adult cell proliferation have been investigated in the
hippocampal formation in rodents as well as other mammalian models, but there has been limited
research conducted in the non-human primate. In the present study, the long-term effects on cell
proliferation of selective brain lesions, produced as part of a separate behavioral study, were
evaluated in the dentate gyrus (DG) of the hippocampus. A total of twelve young male monkeys
(3.9-7.9 years of age) were studied. Six animals received aspiration lesions of the hippocampus
in one hemisphere and the other six animals were unoperated controls. Each animal received
intraperitoneal injections of the synthesis phase marker 5-bromo-2‟-deoxyuridine (BrdU), 21-22
days prior to perfusion-fixation of the brain which occurred 7 to 18 months after the lesion. After
immunohistochemical processing, BrdU positive cells in the DG were quantified in the intact