[Abstract Title]. - Society for Neuroscience

Authors: Y. JANG, H. CHO, H. KIM, Y. YANG, S. PARK, M. TAK, T. KIM, Y. CHO, *U.
OH;
Sensory Res. Ctr., Seoul Natl. Univ. Col. Pharm, Seoul 151 742, Republic of Korea
Abstract: Neurogenesis and plasticity of neurons are correlated with neurite outgrowth. Recently
the importance of transient receptor potential (TRP) channels for the neuronal differentiation and
proliferation has been exhibited. But, it is still not well understood which types of TRP channels
are involved in neurite outgrowth. So we screened types of TRP channels are required for the
neuronal outgrowth, using real time quantitative-PCR technique in PC12 cells. As a result, we
hypothesized that TRPM2 is related to neurite outgrowth.
TRPM2 is highly expressed in the brain, but its role in the nervous system is largely unknown.
We report that its expression with small interfering RNA increased neurite outgrowth in live cell
imaging. Its overexpression decreased the length of neurite. Also the length of neurite is
increased by its inhibitors such as econazole, clotrimazole and flufenamic acid. These results
suggest that TRPM2 inhibits nuerite outgrowth as negative regulator. We found that expression
of TRPM2 and LPA receptor is colocalized in the neuronal cell. Actually, LPA-induced Ca 2+
influx is higher in TRPM2 overexpressed in PC12 cell than those in control, using whole-cell
recording and Ca 2+ imaging. Furthermore, LPA-induced neurite retraction is recovered by
blocking TRPM2 channel. Therefore These findings suggest that TRPM2 inhibits neurite
outgrowth via LPA receptor.
Disclosures: Y. Jang, None; U. Oh , None; H. cho, None; H. kim, None; Y. Yang, None; S.
park, None; M. Tak, None; Y. cho, None; T. kim, None.
Poster
265. Nociceptors II
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 265.20/EE21
Topic: B.02.t. TRP Channels
Title: Use of laser Doppler imaging in rats to evaluate activation of Transient Receptor Potential
channels in rats
Authors: V. A. KENIGS, *M. R. BRANDT, C. M. FLORES;
Analgesics Drug Discovery, Johnson & Johnson Pharmaceut R&D, Spring House, PA
Abstract: Transient Receptor Potential (TRP) channels are a family of non-selective cation
channels having a multitude of physiologic functions such as sensory transduction, taste, and
thermosensation. In recent years, many naturally occurring chemicals have been identified that