[Abstract Title]. - Society for Neuroscience

educed to 60%, 76%, and 56% of control levels in the 14-3-3ζ, 14-3-3ε, and 14-3-3γ lines,
respectively. Cells overexpressing 14-3-3β or 14-3-3δ were only mildly resistant to similar doses
of rotenone and did not show a significant reduction of MPP+-induced cell death as compared to
control. Cell lines overexpressing 14-3-3ε or 14-3-3ζ showed no significant reduction of cell
death in response to rotenone or MPP+ but instead showed a trend of increased toxicity.
We are currently evaluating whether 14-3-3ε, γ, or ζ can reduce the dopaminergic cell loss seen
in transgenic C. elegans that overexpress α-syn. Preliminary results of transgenic worms
overexpressing 14-3-3ζ show a reduction in dopamine cell loss. Our findings suggest that certain
14-3-3 proteins may serve as potential targets for neuroprotective therapy in PD.
Disclosures: T.A. Yacoubian , None; S.R. Slone, None; S. Hamamichi, None; A.J.
Harrington, None; K.A. Caldwell, None; G.A. Caldwell, None; D.G. Standaert, None.
Poster
247. Parkinson's Disease Interventions: Animal and Clinical Models
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 247.19/T2
Topic: C.02.d. Therapies
Support: PDF
NS26836
DAMD17-03-1-0492
NS38370
Title: Restoration of the nigrostriatal dopaminergic pathway in mouse models of Parkinson‟s
disease: effects of a constitutively active form of AKT
Authors: *X. CHEN 1 , T. KAREVA 1 , M. RZHETSKAYA 1 , C. WANG 3 , M. DURING 3 , N.
KHOLODILOV 1 , R. E. BURKE 1,2 ;
1 Dept Neurol, 2 Pathology and Cell Biol., Columbia Univ., New York, NY; 3 Human Cancer
Genet. Program, The Ohio State Univ., Columbus, OH
Abstract: Restoration of the nigrostriatal dopaminergic projection and reinstatement of
dopamine function presents one of the greatest challenges for the development of new
therapeutic strategies for Parkinson‟s disease (PD). We have previously reported that
myristoylated Akt (Myr-Akt), a constitutively active form of Akt, has striking neuroprotective