[Abstract Title]. - Society for Neuroscience

(astrocytes/Type 1 neural progenitors) or NeuN (mature neurons) in vitro and in vivo.
Incorporation of 5-bromo-2‟-deoxyuridine (BrdU) was used to assess cell proliferation in Cx29
+/+ and -/- mice and postnatal neural progenitor neurosphere cultures. Both genotypes had
comparable numbers of BrdU+ cells in the SVZ and SGZ in vitro and in vivo. In addition to
BrdU labelling, we used the mitotic marker phospho-histone H3 to assess the mitotic index of
our postnatal neurosphere cultures and again found no significant difference between
neurospheres derived from +/+ and -/- animals. In sum, Cx29 was found to localize to a small
subset of PDGFαR expressing early oligodendrocyte progenitor cells in both neurogenic niches
of the mouse brain but was not required for cell proliferation in these areas.
Disclosures: S. Imbeault, None; V. Peshdary, None; H. Toeg, None; S.A.L. Bennett, None.
Poster
241. Glial Neuronal Interactions
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 241.22/H4
Topic: C.10.a. Brain: Cellular and molecular mechanisms
Support: National Basic Research Program (G 1999054005)
National Natural Science Foundation (39930090, 39870248)
Title: The expression changes of Neuropilin-1 and its ligand Sema3A and VEGF in the
hippocampus following entorhinal deafferentation
Authors: *W. WANG 1,2 , C. ZHOU 2 ;
1 Picower Inst., MIT, Cambridge, MA; 2 Shanghai Inst. for Biol. Sciences, Chinese Acad. of Sci.,
Shanghai Inst. of Physiol., Shanghai, China
Abstract: Neuropilin-1 (NRP-1) is a cell surface receptor shared by class3 semaphorins and
vascular endothelial growth factor (VEGF), and plays multiple roles through different signal
pathways by interacting with these two ligand families. Moreover, it has been shown that VEGF
could antagonize the Sema3A induced apoptosis and growth cone collapse by competing with
Sema3A for binding to NRP-1. Here we reported the expression changes of NRP-1 and its ligand
VEGF and Sema3A in the mouse hippocampus after transections of the entorhinal afferents.
Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) analysis showed the
significant expressed up-regulation of NRP-1 and VEGF mRNAs in the deafferented
hippocampus with its maximal elevation at 7-15 days postlesion while the Sema3A mRNA level
remained unchanged at any time point we examined. In situ hybridization and