[Abstract Title]. - Society for Neuroscience

memory circuitry by ketamine that is consistent with human data and a critical interaction
between a D1 agonist and ketamine which is targeted towards dlPFC in the primate.
Disclosures: G.V. Williams , Pfizer Global Research and Development, B. Research Grant
(principal investigator, collaborator or consultant and pending grants as well as grants already
received); B.M. Roberts, None; D.E. Holden, None; D.W. Campbell, None; D.P.
Weinzimmer, None; C.M. Sandiego, None; R.E. Carson, None; S.A. Castner, Pfizer Global
Research and Development, B. Research Grant (principal investigator, collaborator or consultant
and pending grants as well as grants already received).
Poster
296. Learning and Memory: Physiology and Imaging III
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 296.10/TT16
Topic: F.02.i. Learning and memory: Physiology and imaging
Support: FNRS grant 3100AO-108336/1 and /2 to PJM
Title: JULIDE : a new software for the unbiased analysis and 3D reconstruction of brain
autoradiograms
Authors: *J. PARAFITA 1 , F. MAGARA 3 , R. CHARRIER 2 , D. RIBES 2 , J.-P. THIRAN 2 , P.
MAGISTRETTI 1,3 ;
1 SV-BMI, 2 STI-LTS5, EPFL, Lausanne, Switzerland; 3 Ctr. for Psychiatric Neurosciences,
CHUV, Lausanne, Switzerland
Abstract: The 14 C-2 Deoxy-D-Glucose (2DG) autoradiographic technique affords a valuable
means to assess in laboratory animals the engagement of brain regions and circuits in a given
behavioural task or following a pharmacological intervention, by indirectly determining glucose
utilization (CMRGlu) associated with neuronal activity (Sokoloff et al 1977). One of the
drawbacks of the technique is that it implies an a priori definition of regions of interest (ROI); in
addition 3D reconstructions of the whole brain with unbiased identification of activated (or
deactivated) regions would be highly desirable, as currently achieved in human functional
imaging studies. Recent attempts in this direction have been proposed (Dubois, A et al 2008).
Here we report the development of a novel approach to achieve such goals, based on merging
and warping of brain autoradiograms prepared from mice belonging to the same experimental
group, and aimed at the unbiased identification of regions of interest combined with 3D
reconstruction.
JULIDE is a software that allows users to detect activated areas in the brain by performing a