[Abstract Title]. - Society for Neuroscience

induced by intracolonic injection of deoxycholic acid. Intrathecal administration of gabazine to
the TL segments significantly increased the VMR while having no effect when administered to
the LS segments in normal rats. In the presence of chronic colonic inflammation, intrathecal
administration of gabazine to the TL or LS segments significantly decreased the VMR. Spinal
administration of gabazine significantly increased the response of TL dorsal horn neurons to
noxious CRD in normal rats, but significantly decreased the response of TL dorsal horn neurons
in chronic colonic inflamed rats. Spinal administration of gabazine had no effect on the response
of LS dorsal horn neurons to noxious CRD in both normal and colonic inflamed rats. These
results suggest that GABA-A receptors contribute to the differential processing of visceral
nociception in TL and LS spinal cord segments.
Disclosures: B. Tang, None; Y. Ji, None; M.S. Gold, None; R.J. Traub , None.
Poster
269. Pain: Visceral Pain II
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 269.18/GG33
Topic: D.08.n. Visceral pain
Support: POCTI/SAU-NEU/55983/2004, FCT, Portugal.
Title: BDNF is important for bladder hyperactivity and pain in a rat model of chronic bladder
inflammation
Authors: *C. CRUZ 1 , R. PINTO 1,2 , S. ALLEN 3 , D. DAWBARN 3 , S. MCMAHON 4 , F.
CRUZ 1,2 ;
1 Inst. Histology and Embryology,, Fac Med, IBMC, Univ. of Porto, Portugal, Porto, Portugal;
2 Dept. of Urology, Hosp. de S.Joao, Porto, Portugal; 3 Mol. Neurobio. Unit, Univ. of Bristol,
CSSB, Dorothy Hodgkin Building, Bristol, United Kingdom; 4 Neurorestoration Group, London
Pain Consortium, King's Col. London, Wolfson CARD, Wolfson Wing, London, United
Kingdom
Abstract: Brain derived nerve factor (BDNF) is a trophic factor belonging to the neurotrophin
family that is upregulated in various inflammatory conditions, where it may contribute to altered
pain states. In the case of bladder inflammation, little is known about the relevance of BDNF in
bladder pain and hyperactivity, a matter we aimed to clarify in the present study.
Female rats (n=4/group) were treated with cyclophosphamide (CYP; 200 mg/Kg). For a period
of 3 days, animals received daily injections in the tail vein of sterile saline or TrkB-Ig2 (100 or
200 ug). TrkB-Ig2 is a recombinant immunoglobulin-like domain that binds to BDNF, with