[Abstract Title]. - Society for Neuroscience

upper jaws were processed for immunocytochemistry for CAV-1, -3 and PMCA at the levels of
light and electron microscopy. Some immunostained sections were treated with histochemistry
for non-specific cholinesterase (nChE) activity. The periodontal Ruffini endings were
immunopositive for Cav-1, but not Cav-3. In immunoelectron microscopy, immunoreactive
products for Cav-1 were confined to caveolae in the cell membrane of the cytoplasmic
extensions and cell bodies of the terminal Schwann cells associated with the periodontal Ruffini
endings. However, the axonal membranes of the terminals did not express any Cav-1
immunoreaction. Immunocytochemistry for PMCA demonstrated intense immunoreaction in the
periodontal Ruffini endings. Double staining with PMCA and PGP 9.5 disclosed the colocalization
of immunoreactions in the axonal branches of the periodontal Ruffini endings, but
not in the nerve bundles in the periodontal ligament. Double labeling with PMCA and S-100
protein showed the lack of immunoreaction for PMCA in the terminal Schwann cells. Since Ca 2+
plays important role in mechanotransduction, these characteristic immunolocalization shows
Cav-1/ Ca 2+ -ATPase might be involved in quick elimination of intracellular Ca 2+ in
mechanotransduction.
Disclosures: A. Suzuki, None; K. Nozawa-Inoue, None; Y. Kawano, None; H. Ajima,
None; T. Maeda , None.
Poster
271. Mechanoreceptors
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 271.5/HH24
Topic: D.09.a. Cellular and molecular mechanisms of transduction
Support: NIH Grant NS036816
Title: Rapid adaptation in the Pacinian corpuscle is partially due to GABAergic inhibition
emanating from the capsular lamellar cells
Authors: *L. J. PAWSON 1 , L. T. PRESTIA 2,1 , G. K. MAHONEY 1 , B. GUCLU 3,1 , P. J.
COX 2,1 , A. K. PACK 2,1 ;
1 Biomed & Chem Eng, Syracuse Univ., Syracuse, NY; 2 Biol., Utica Col., Utica, NY; 3 Biomed
Eng Inst., Bogazici Univ., Istanbul, Turkey
Abstract: The Pacinian Corpuscle (PC) is an onion-shaped cutaneous tactile mechanoreceptor,
which rapidly adapts to sustained pressure (indentation, displacement or force). The receptor
consists of a nerve ending (neurite), which is encapsulated first by inner-core hemi-lamellae that
develop from Schwann cells, and then by outer-core lamellar cells that originate from fibroblasts.