[Abstract Title]. - Society for Neuroscience

located in the superficial mantle of the lateral and medial ganglionic eminences (LGE and MGE)
between E11.5 and E13.5. The I56ii-positive cells are Dlx- and GABA-immunoreactive.
However, unlike the other CREs, I56ii does not label interneuron progenitors in the basal
ganglia, nor tangentially migrating cells to the cortex at E13.5. Instead, I56ii-positive cells mark
a subpopulation(s) of post-mitotic projection neurons that tangentially migrate from the LGE to
the deep mantle of the MGE and reside between the subventricular zone and the globus pallidus
during midgestation. The majority of these neurons express the striatal markers Meis2 and Islet1.
Moreover, both Meis2 and Islet1 activate transcription of a reporter gene containing the I56ii
sequence in co-transfection assays, indicating that these transcriptional factors may be potential
upstream modulators of the Dlx genes in vivo. In summary, our data suggest a complex and
dynamic regulation of Dlx gene expression during early stages of embryonic development
through several regulatory elements with overlapping and distinct function(s).
Disclosures: M. Yu, None; N. Ghanem, None; L. Poitras, None; J.L.R. Rubenstein, None; M.
Ekker, None.
Poster
230. Cell Migration: Molecules Mediating Migration
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 230.21/B11
Topic: A.02.c. Cell migration
Title: On the chronological course of zinc distribution in the developing mouse cerebellum
Authors: *M. BORGMANN 1 , S. FRISCHMUTH 2 , J. BAEURLE 2 ;
2 Physiol., 1 Charite - Berlin, Berlin, Germany
Abstract: Early development and normal formation of the cerebellum critically depends on zinc
finger transcription factors. In the mature brain, Zn is considered to act as a co-transmitter and
neuromodulator in certain brain regions. Moreover, elevated concentrations of Zn in
neurodegenerative diseases have also suggested a role for Zn in neuronal cell death. Thus, Zn
seems to be involved in a number of essential functions during different phases of brain
development and maturation.
However, only little is known about the spatio-temporal distribution or function of Zn during
normal postnatal cerebellar development.
Here we show the first chronological course of Zn distribution in the mouse cerebellum using
immersion autometallography (AMG), a well established technique to visualize Zn in biological
tissue.
C57Bl/6J mice were sacrificed at postnatal day (P) 7, 14, 19, 22, 24, 29, 83 and 120. Brains were