[Abstract Title]. - Society for Neuroscience

the cyclooxygenase-2 (Cox-2) gene (two-fold) in the hypothalamus, the hunger and satiety center
of the brain, of tumor-bearing mice, as shown by quantitative real-time PCR. The Cox-2 protein
was found to be induced in cells lining the cerebral blood vessels, suggesting a tumor-induced
signalling via brain vascular cells to the brain. As a next step, tumor-bearing mice were given the
Cox-inhibitor indomethacin in the drinking water (6.7 κg/ml) during ten days, which completely
abolished the development of anorexia. This treatment also resulted in reduced tumor growth as
shown previously (Cahlin et al., Cancer Res 60: 1742-9, 2000). To differentiate between the
effect of reduced tumor burden and the Cox-inhibition on the food intake, indomethacin was
given to tumor-bearing mice before lights out at day 7, during established anorexia. The results
show that a single day of treatment restored food intake to normal levels in tumor bearing mice.
These findings indicate that cancer-associated anorexia in this model occurs by inflammatory
mechanisms that involve cyclooxygenases, and show that the beneficial effect of indomethacin
on food intake is not a consequence of reduced tumor size.
Disclosures: J.P. Ruud, None; W. Wang, None; K. Lundholm, None; A. Blomqvist, None.
Poster
284. Regulation of Food Intake and Body Weight: Integration of Peripheral Signals:
Systems
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 284.21/QQ16
Topic: E.07.b. Integration of peripheral signals: Systems
Title: Putative flip-flop regulation of running and eating in rats
Authors: *M. BANNAI 1,2 , K. KASUH 2 , K. NAKAHARA 2 , N. MURAKAMI 2 , M.
TAKAHASHI 1 ;
1 Inst. of Life Sci., Ajinomoto Co., Inc., Kawasaki-shi, Japan; 2 Dept of Vet. Physiol, Univ. of
Miyazaki, Miyazaki, Japan
Abstract: Rats run up to 10 km/day if they are kept in the cage equipped with a running wheel.
Running and eating never occur simultaneously suggesting a substantial flip-flop regulation. We
have previously reported interaction between wheel running and food consumption where food
consumption decreased either slightly or significantly by deprivation of a running wheel or
giving “re-run” condition, respectively. In this study, daily running period was restricted in order
to know the implication of “running deprivation”. Male Wistar rats were housed individually in a
cage with running wheel and food consumption was recorded daily. The controls were kept in a
cage without a running wheel. The experimental group was allowed to access freely to the wheel
from day 1 to 19 (unbound-running-1), access to the wheel only 3 hours in the dark phase from