[Abstract Title]. - Society for Neuroscience

control of NREM sleep. In e5NT KO mice, homeostatic sleep pressure accumulates normally
and may not depend on rising adenosine levels. However, the impaired recovery sleep in these
mice suggests that increases in adenosine, mediated by e5NT, are necessary for the normal
recovery of NREM sleep.
Disclosures: T. Mochizuki, None; E.L. Clark, None; L.F. Thompson, None; T.E. Scammell,
None.
Poster
286. Imaging the Nervous System
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 286.1/QQ41
Topic: E.09.e. Functional imaging
Support: ANR-06-NEURO-004-01 Astroglo
Title: Intrinsic and autofluorescent optical signals and the neuro-astrocytic activities in the
rodent olfactory glomeruli
Authors: *H. GURDEN, B. L'HEUREUX, F. LEFEBVRE, L. PINOT, B. JANVIER, R.
MASTRIPPOLITO, P. LANIECE, F. PAIN;
IMNC, CNRS UMR8165, Orsay, France
Abstract: Changes in the neuro-glial activity induce variations in optical properties of brain
tissues. Imaging of Intrinsic Optical Signals (IOS) allow mapping of sensory-evoked activity but,
because they are due to local blood-related changes, these signals are indirectly linked to neural
activity. We use them in the rodent olfactory bulb (OB) to map odor-induced activation of
olfactory glomeruli. We previously studied the cellular triggers of odor-induced IOS in rats and
reported that glutamate release and uptake through astrocytic transporters are at their origin. In
this context, we are developing our research following two complementary ways.
i- Further studies of IOS triggers. According to our preliminary results, 2 DeoxyGlucose uptake
is impaired in GLAST-Knock Out (KO) but not in GLT1-KO mice (see poster of Claire Martin
in this meeting). We observed that GLAST-KO mice present intact glomerular IOS maps for a
given odorant. To probe the dose-response curves between KO and wild-type, we are currently
comparing odor-induced IOS intensity for several odor concentrations.
ii- Recording autofluorescence (AF) signals from the intracellular compartment. This technique
relies on AF properties of flavoproteins. To evaluate the feasibility of AF recording in the OB,
we performed Monte Carlo simulations of photons travel through biological tissues. We
implemented a multi-layer model for the OB but also for the somatosensory cortex that we used