[Abstract Title]. - Society for Neuroscience

the midline. Intriguingly, MsSrc may also integrate responses to other pathway cues encountered
by migrating neurons in ENS, including attractive responses to the adhesion receptor Fasciclin II.
We are currently using in vivo and in vitro methods to further characterize the signaling
pathways by which input from multiple guidance cues converge to regulate neuronal guidance in
the developing ENS.
Disclosures: P.F. Copenhaver , None; T.M. Coate, None; T.L. Swanson, None.
Poster
230. Cell Migration: Molecules Mediating Migration
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 230.17/B7
Topic: A.02.c. Cell migration
Title: Migration of stellate/basket cells in developing cerebellar cortex
Authors: *H. KOMURO 1 , D. B. CAMERON 1 , Y. JIANG 1 , K. KASAI 2 , Y. SAEKI 2 ;
1 Dept Neurosci, NC30, Lerner Res. Inst. Cleveland Clin. Fndtn, Cleveland, OH; 2 Neurolog.
Surgery, The Ohio state Univ., Columbus, OH
Abstract: The precursors of cerebellar stellate/basket cells reside in the deep WM, but little is
known about how these neurons attain their final positions within the ML. In this study, with the
use of a HSV amplicon plasmid pHGY, which expresses EGFP under the control of the HSV
IE4/5 promoter, we examined the migration of stellate/basket cells in the early postnatal mouse
cerebellum. We found that stellate/basket cells sequentially go through four distinct phases of
migration. The first phase (Phase I) is a period of when basket/stellate cells migrate radially from
the bottom of the IGL through the PCL towards the top of the ML. Upon reaching the top of the
ML, basket/stellate cells undergo the first change of the direction of migration from radial to
tangential. The second phase (Phase II) is a period of when basket/stellate cells migrate
tangentially in the rostro-caudal direction (perpendicular to the folia) at the top of the ML.
Thereafter, basket/stellate cells undergo the second change of the direction of migration from
tangential to radial. The third phase (Phase III) is a period of when basket/stellate cells migrate
radially within the ML from the top to the bottom and vice versa with multiple branches of the
leading processes. Subsequently, basket/stellate cells undergo the third change of the direction of
the migration from radial to tangential in the rostro-caudal direction at the middle and the top of
the ML. The fourth phase (Phase IV) is a period of when basket/stellate cells tangentially migrate
in the rostro-caudal direction and complete their migration at the middle and the top of the ML.
Stellate/basket cells exhibited the distinctive rate of migration during each phase of migration.
The average rates of migration were ~14.9 κm/h in Phase I, ~15.4 κm/h in Phase II, ~7.2 κm/h in