[Abstract Title]. - Society for Neuroscience

acetylcholine. These results suggests unique properties of (+/-)TC-5619 with respect to α7
nAChR desensitization when compared with other selective α7 agonists.
Disclosures: F.W. Jow , None; M. Bowlby, None; T. Lock, None; R. Peri, None; D. Kowal,
None; A. Nencini, None; S. Haydar, None; C. Ghiron, None; G. Tertsappen, None; J.
Dunlop, None.
Poster
233. Nicotinic Aacetylcholine Receptors: Regulation and Function II
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 233.6/C8
Topic: B.02.a. Nicotinic acetylcholine receptors in brain: Physiology and function
Support: Barrow Neurological Foundation
Philip Morris USA Inc. and Philip Morris International through their External
Research Program
NIH U19 DA019377
NIH R01 DA017980
NIH R01 DA015389
Title: Comparisons across techniques used to characterize the functional pharmacology of
ligand-gated ion channels: Toward a universal scale of function
Authors: B. EATON 1 , Q. LIU 2 , C. ZHENG 2 , B. DASH 1 , J. WU 2 , *R. J. LUKAS 1 ;
1 Div. Neurobiol, 2 Div. Neurol., Barrow Neurol Inst., Phoenix, AZ
Abstract: With the diversity of techniques available for characterizing the functional
pharmacology of ligand-gated ion channels, there is a need to establish an objective means to
compare data obtained from different labs using these different techniques and expression
systems. As high-throughput techniques become more affordable and prevalent and novel
techniques are created, this need will only increase. Potentially, even data obtained using the
same technique in cells transfected with identical DNA constructs might differ subtly if the host
cell line differs. Here we compare functional data for nicotinic acetylcholine receptor (nAChR)
subtypes expressed in oocytes or mammalian cells and assessed using whole-cell current
recording, isotopic ion flux assays, or fluorescence-based assays using voltage-sensitive dyes.