[Abstract Title]. - Society for Neuroscience

to wide-band noise at the level of 120 dB for 3 hours/day for two consecutive days, we found
that the CLB was collapsed. Serum proteins such as H and L chains of IgG and albumin become
present in the stria vascularis. The instrastrial space was enlarged. Basement membrane between
the pericyte and the endothelial cell was discontinuous at some points. Pericytes also showed
multiple abnormalities: (1) Pericytes had an abnormally loose association with endothelial cells,
with wide spaces separating some regions of two types of cells. In some cases, pericytes‟ long
processes extended away from the vessel wall; (2) The amount of pericytes structural protein,
desmin, was substantially increased by 39 % (~12% in normal condition) in the stria vascularis;
its mRNA was increased by 6.79 ± 2.6 fold; (3) Infiltration of GFP + bone marrow-derived cells
(GFP + -BMDCs) was observed in the areas of spiral ligament. Some of GFP + -MBDCs expressed
the pericyte marker protein, desmin, at the sites on or close to vessels. These observations
indicate that the abnormal relationship of pericytes with endothelial cells may influence the
endothelium and contribute to the leakness after sound trauma. The increase of pricytes structural
protein and pericyte recruitment may suggest an early response for maintaining CBL. This work
was supported by NIH R03 DC 008888.
Disclosures: X. Shi , Oregon Hearing Research Center (NRC04), A. Employment (full or parttime);
Department of Otolaryngology and Head & Neck Surgery, Oregon Health & Science
University, 3181 SW Sam Jackson Park Road, Portland, OR 97201-3098. USA, A. Employment
(full or part-time).
Poster
287. Blood Brain Barrier and CSF
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 287.6/QQ60
Topic: E.09.c. Blood brain barrier
Title: Assessment of blood-brain barrier damage by microdialysis probe implantation
Authors: *R. K. SUMBRIA, U. BICKEL;
TTUHSC, Amarillo, TX
Abstract: Intracerebral microdialysis is increasingly used as a technique to determine the
pharmacokinetics of drug uptake by brain. However, it is an invasive technique, and it is crucial
to answer the question whether blood-brain barrier (BBB) integrity is maintained after probe
implantation before applying microdialysis as a quantitative tool. Studies done to date gave
contradicting results, which may in part be due to technical variation in probe implantation and
in the lag time of the dialysis sampling. We are using a microdialysis technique, which should
result in the lowest possible BBB damage, because dialysate measurements are performed 24hr