[Abstract Title]. - Society for Neuroscience

231. Dendrite Growth and Branching: Signaling
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 231.16/B29
Topic: A.04.i. Dendritic growth and branching
Support: 21st Century Frontier Research Program Grant M103KV010023-07K2201-02510
Title: Role of inositol 1,4,5-trisphosphate 3 kinase-A in neuronal development
Authors: S. HONG, I. KIM, B.-I. CHOI, S. HAN, J. MO, I. RHYU, W. SUN, *H. KIM;
Dept Anat., Korea Univ. Col. Med., Seoul, Republic of Korea
Abstract: The inositol 1,4,5-trisphosphate 3 kinases (IP3Ks) are known as regulators for the
maintenance of intracellular calcium homeostasis. Among three IP3K isoforms (IP3K-A, B and
C), IP3K-A appears to play a significant role in neuronal development, because it exhibits
developmentally regulated neuron-specific expression. However, the precise molecular function
of IP3K-A in neuronal development is currently unknown.
In this study, we investigated the developmental expression pattern of IP3K-A in the rat brain
with in situ hybridization histochemistry, and the roles of IP3K-A in neuronal development with
gene perturbation experiments.
The expression of IP3K-A was observed from neonate and gradually increased during the
postnatal brain development. Spatially, strong expression was found in the hippocampal
formation, cerebral cortex, caudate putamen and cerebellum. Over-expression of IP3K-A in
primary cultured hippocampal and cerebral cortical neurons increased the number and length of
dendritic protrusions compared to the control neurons. Conversely, suppression of IP3K-A with
siRNA in primary cultured hippocampal neurons retarded the development of dendritic
protrusions. Similar deficits in the dendritic spine development were also found in the
hippocampus of IP3K-A knockout mice.
These results suggest that IP3K-A plays a significant role in the development of dendritic spines.
Disclosures: S. Hong, None; I. Kim, None; B. Choi, None; S. Han, None; J. Mo, None; I.
Rhyu, None; W. Sun, None; H. Kim , None.
Poster
231. Dendrite Growth and Branching: Signaling
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 231.17/B30