[Abstract Title]. - Society for Neuroscience

Abstract: Serotonin (5-hydroxytrypyamune, 5-HT) modulates positively and negatively through
a G-protein-coupled cAMP-mediated pathway. Notably, cAMP production has been linked to
memory formation and multiple 5-HT receptors have been implicated in memory. Hence,
memory formation and cAMP production were investigated in an autoshaping task. The
autoshaping time-course showed that there was a significant increment in the %CR at 48 h
relative to 1.5 and 24 h testing times. Therefore, 24 and 48 h were selected for further memory,
pharmacological and cAMP production experimental analysis. Notably, 24 and 48 h allow
detecting upwards or downwards changes about cAMP and memory, thus providing an excellent
window to determine how they are related. Even to further test this link, an overtraining group
showing 100% of CR was included. The post-training administration of the 5-HT1A/7 receptor
agonist 8-OH-DPAT or 5-HT7 receptor agonist AS19 improved memory, which were blocked by
the 5-HT1A receptor antagonist WAY100635 or 5-HT7 receptor antagonist SB-269970. Changes
in cAMP production in prefrontal cortex, hippocampus and raphe nuclei were associated to
memory formation, pharmacological manipulation and amnesia. Indeed, untrained animals
treated with saline, 8-OH-DPAT, AS19, scopolamine and dizocilpine showed changes in cAMP
production ranging between 100 to 2000%, and similar but trained animals had changes varying
between 100 to 500%. Together these data show for the first time, serotonergic modulation of
memory formation and cAMP production. And this evidence is consistent with the notion that
modulation of cAMP-dependent pathways is viable therapeutic tool in of cognitive impairment.
Disclosures: G.P. Garcia , None; A. Meneses, None.
Poster
290. Animal Cognition and Behavior: Learning and Memory: Pharmacology I
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 290.21/RR72
Topic: F.02.j. Learning and memory: Pharmacology
Support: NARSAD Toulmin Independent Investigator Award
NIH Grant MH73725
Title: Perinatal choline supplementation improves hippocampal-based learning and memory in
the DBA/2 mouse
Authors: *K. E. STEVENS 1,2 , R. C. BATES 2 , J. GAULT 3 ;
1 Med. Rsrch, Veterans Affairs Med. Ctr., Denver, CO; 2 Psychiatry, 3 Neurosurg., Univ. of
Colorado-Denver, Sch. of Med., Aurora, CO