[Abstract Title]. - Society for Neuroscience

voxel-based statistical analysis. It is fully written in ITK (National Library of Medicine Insight
Segmentation and Registration Toolkit) and presents a user-friendly interface.
JULIDE is based on a four-step approach which includes: (1) an automated pre-processing to
select the desired section, (2) a 3D reconstruction of autoradiographic volumes, (3) a spatial
normalization to compensate all the differences in position, orientation and shape and finally (4)
a 3D voxel-based statistical analysis based on t-test.
Outputs of JULIDE are two 3D activation maps allowing to identify the significant differences
(positive and negative) in CMRGlu between two groups. The comparison of CMRGlu between
two groups is represented by color-coded statistical maps of p values defined by the user.
We have applied this approach to the study of the regional variations in CMRGlu in mice
undergoing a spatial learning task. Significant differences in the areas engaged during the
behavioural task at day 1 vs day 9, when animals are highly performing, have been identified .
Results obtained with JULIDE are comparable to those obtained manually by optical
densitometry of pre-selected ROI such as the hippocampus, the most activated region at day 1.
This metabolic activation is localized to the cingulate and retrosplenial cortices at day 9.
Softwares such as JULIDE and other recently proposed (Dubois, A et al 2008) are likely to
provide useful tools to exploit to its full extent the use of 2DG autoradiography to complement
behavioural and pharmacological studies in laboratory animals.
Disclosures: J. Parafita , None; F. Magara, None; R. Charrier, None; D. Ribes, None; J.
Thiran, None; P. Magistretti, None.
Poster
296. Learning and Memory: Physiology and Imaging III
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 296.11/TT17
Topic: F.02.i. Learning and memory: Physiology and imaging
Support: NIMH Grant MH-072641
Sigma Xi Grants-in-Aid of Research
NASA GSRP Grant NNX06AH25H-S01
Title: Hemodynamic and electrophysiological evidence of resting-state network activity in the
primate