[Abstract Title]. - Society for Neuroscience

Poster
269. Pain: Visceral Pain II
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 269.13/GG28
Topic: D.08.n. Visceral pain
Support: POCTI/SAU-NSE/55983/2004
Title: Human urothelial cells respond to inflammatory mediators trough a TRPV1-dependent
mechanism
Authors: *A. A. SILVA 1 , A. CHARRUA 1 , C. REGUENGA 1 , J. M. CORDEIRO 2 , M.
DUARTE-ARAÚJO 2 , P. CORREIA-DE-SÁ 2 , C. PAULE 3 , I. NAGY 3 , F. CRUZ 4 ;
1 Inst. of Hist and Embryol, Fac Medicine, IBMC, Univ. of Porto, Portugal, Porto, Portugal;
2 Laboratório de Farmacologia/UMIB, ICBAS, Porto, Portugal; 3 Imperial Col. London, London,
United Kingdom; 4 Inst. of Hist and Embryol, Hosp. de São João, IBMC, Univ. of Porto,
Portugal, Porto, Portugal
Abstract: Human urothelial cells express the TRPV1 receptor. Recent works have indicated that
TRPV1 might be responsible for the control of pain and micturiction control during chronic
cystitis. Here, we studied the functionality of the TRPV1 channel in cultured human urothelial
cells. For that, we performed cobalt uptake experiments, an indirect method to measure the
calcium influx into cells, in cells exposed to normal media, to a solution composed of
inflammatory mediators and a solution composed of inflammatory mediators and capsazepine (a
TRPV1 antagonist). Our data shows that inflammatory mediators induced a TRPV1-dependent
increase of cobalt uptake, since this effect was completely blocked by capsazepine. Previous
results from our group have shown that capsaicin induces cobalt uptake from cultured human
urothelial cells, an effect completely reverted by capsazepine. In order to understand the
consequence of human urothelial TRPV1 activation, we analysed by luminometry the capsaicininduced
ATP release. Capsazepine significantly inhibited the release of ATP by the urothelial
cells, thereby implicating TRPV1 in ATP secretion from human urothelium. This work shows
further evidence that TRPV1 expressed in human urothelial cells have an important role in
micturition control under pathological conditions such as cystitis.
Disclosures: A.A. Silva, None; A. Charrua, None; C. Reguenga, None; J.M. Cordeiro,
None; M. Duarte-Araújo, None; P. Correia-de-Sá, None; C. Paule, None; I. Nagy, None; F.
Cruz, None.