[Abstract Title]. - Society for Neuroscience

humans. Citalopram binds to the SERT and blocks the reuptake of the serotonin into the synaptic
terminals. The aim of this study was to assess the effect of citalopram treatment on the density of
the SERT in FSL (FSL-CTP group) and SD (SD-CTP) rats [14-day therapy; 10 mg/kg/day of
citalopram delivered by the osmotic minipump]. The controls received saline (FSL-SAL and SD-
SAL groups). Following the treatment, the rats were decapitated and the brains were frozen in
isopentane and stored at -80º C. The brains were cut in a microtom on slides 20 µm thick. The
binding experiment was performed as described in Psychopharmacology (Berl.) 178 (2005)276-
85 using 20 pM [ 125 I]-(-)-2β-Carbomethoxy-3β-(4-iodophenyl)tropane ( 125 I-RTI-55) with the
presence of 200 nM 1-(2-Diphenylmethoxyethyl)-4-(3-phenylpropyl)piperazine dihydrochloride
(GBR12935) for 120 min. Non-specific binding was measured in the presence of 1 mM of
paroxetine along with 200 nM GBR12935. The slides were contacted with Fuji 14 C imaging
plates along with the 125 I standards for 24 hours and scanned in a Fuji BAAS 5000 scanner. The
images were quantified with the MCID image analyzer calibrated with standards. The regional
values were assessed in 24 brain regions and transformed to receptor concentrations (fmol/mg).
The statistical analysis was performed using STATISTICA software. One-way ANOVA
revealed: 1) significant differences in 5 regions between the FSL-CTP and FSL-SAL groups,
F(2,9)=39.8; p