[Abstract Title]. - Society for Neuroscience

Authors: *Y. RAMIRO 1 , J. MORÁN 2 ;
1 INSTITUTO DE FISIOLOGIA CELULA, MEXICO, D.F., Mexico; 2 INSTITUTO DE
FISIOLOGIA CELULAR, Mexico, d.f., Mexico
Abstract: Apoptotic cell death is an intricate process involving a wide range of signaling
pathways, including the Jun N-terminal Kinases (JNK), a member of mitogen-activated protein
kinases (MAPKs). It has been reported that JNK pathway is activated under stress in many type
of cells. When cerebellar granule neurons (CGN) are cultured in a medium with high potassium
(K25) during 6-8 days in vitro and then transferred to a medium with low potassium (K5) or
staurosporine (Sts), most of the cells die after 24-48 hrs by apoptosis. We reported that apoptosis
induced by K5 and Sts induces an early increase of ROS levels, followed by the activation of the
apoptotic machinery. In the present study we found that the inhibition of JNK activation with
SP600125 prevented caspase 3 activation, nuclear condensation and cell death induced by K5. In
contrast, JNK inhibition did not affect cell death induced by Sts. On the other hand, antioxidants
increased ASK1 phosphorylation and decreased JNK1/2 and c-Jun activation induced by K5.
These results suggest that apoptosis induced by K5 is JNK-dependent and mediated by ROS, but
apoptosis induced by Sts is not dependent of JNK, suggesting that other signaling pathway is
responsible of cell death induced by Sts.
Disclosures: Y. Ramiro, None; J. Morán, None.
Poster
232. Developmental Cell Death: Biological Effects
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 232.14/B54
Topic: A.06.a. Developmental cell death: Biological effects
Support: NIH P20 RR-16460
Title: Rat cortical subplate comparisons across development and aging
Authors: *T. J. TEAGUE-ROSS 1 , S. M. LANTZ 1 , C. M. DAVIS 1 , A. L. JAMES 1 , G. N.
BRATTON 2 , B. CLANCY 1 ;
1 Biol., 2 Mathematics, Univ. Central Arkansas, Conway, AR
Abstract: Subplate neurons participate extensively in the development of the mammalian cortex
and although many undergo apoptosis, a subset remains in adult brains. In conventional models
of development, the persisting subplate cells are considered simply a remnant population.
However, these neurons remain active across aging in advantageous positions in and above the