[Abstract Title]. - Society for Neuroscience

Functional studies showed that HBMVEC cultures expressing CLN-5 under the control of a
VEGF-insensitive promoter were protected from VEGF-induced permeability. Taken together,
these data implicate VEGF-induced downregulation of CLN-5 as an important mechanism in
BBB permeability. They further suggest that loss of the endothelial barrier is accompanied by
formation of an astrocytic organization that may regulate macromolecular traffic in the CNS
parenchyma.
Disclosures: A. Tadesse Argaw, None; G.R. John , None; B.T. Gurfein, None; Y. Zhang,
None; A. Zameer, None; C.F. Brosnan, None.
Poster
252. Demyelinating Disorders: Mechanisms and Therapeutics II
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 252.4/W28
Topic: C.08.a. Molecular and cellular mechanisms
Support: NIH (NS043432)
VA Medical and RR&D Services
NMSS CA1009A10
Title: CNPase expression in olfactory ensheathing cells
Authors: *C. RADTKE 1,2 , K. L. LANKFORD 1 , M. SASAKI 1 , V. GALLO 3 , J. D. KOCSIS 1 ;
1 Yale Univ. Sch. Med., West Haven, CT, CT; 2 Reconstructive Surgery, Hannover Med. Sch.,
Hannover, Germany; 3 Children's Natl. Med. Ctr., Children's Res. Inst., Washington, DC
Abstract: The olfactory bulbs were studied in transgenic mice expressing the enhanced green
fluorescent protein (eGFP) under the control of the 2‟,3‟-cyclic nucleotide 3‟-phosphodiesterase
(CNPase) promoter. CNPase is present in myelinating cells in both the central and peripheral
nervous systems (oligodendroctyes and Schwann cells). eGFP was observed in the nuclei and
cytoplasm of glial cells in the outer nerve layer (ONL) of the olfactory bulb where olfactory
ensheathing cells (OECs) reside, and in oligodendrocytes in the interior of the olfactory bulb.
Immunohistochemical analysis for CNPase in the olfactory bulb demonstrated weak expression
of CNPase in the ONL, but intense expression in oligodendrocytes. However, dissociated OECs
derived from the olfactory bulb and maintained in culture for 4 days had both intense eGFP
expression and CNPase immunoreactivity. These data indicate that CNPase expression of OECs
in vivo is weak, but present as evidenced by accumulated intracellular eGFP in OECs of the