[Abstract Title]. - Society for Neuroscience

to assess changes in gene transcript levels. We have seen successful HDAC2 inhibition between
1-8hr following 20nM dose, and between 24-48hr following 40nM dose. Consequently, we
found decreased BDNF transcript levels between 3-24hr that was normalized at 48hr following
silencing with the 20nM dose of siRNA. Associated increase in TrkB receptor transcript levels
were observed between 8-48hr. Interestingly we also found increased TNF-a transcript levels
between 6-12hr following 20nM silencing. These findings suggest that HDAC2 can regulate
gene transcription for neurotrophic factors and receptors such as BDNF and TrkB in the
hippocampal hilus, which may be associated with mossy fibre sprouting in response to repeated
nicotine in the HR phenotype. Other members of the neurotrophic factor family such as nerve
and fibroblast growth factors are investigated in phenotype-specific link to chromatin remodeling
in nicotine sensitization. Silencing of HDAC2 appear to be associated with increased
inflammatory response, and implications of phenotype- and/or nicotine-specific regulation of
inflammation are discussed. This work is supported by the Florida Department of Health 05NIR-
594 to Dr. Isgor.
Disclosures: O. Oztan , None; C. Aydin, None; A. Bhatti, None; D. Tobiansky, None; C.
Isgor, None.
Poster
258. Molecular and Neurochemical Basis of Nicotine Addiction
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 258.7/AA10
Topic: C.16.k. Nicotine
Support: NIH Grant DA 09407
NIH Grant DA 00378
Title: Nicotine-induced changes in globus pallidus met-enkephalin systems are mediated by
nicotinic and dopamine d-2 receptor mechanisms
Authors: *M. E. ALBURGES, P. S. FRANKEL, A. J. HOONAKKER, G. R. HANSON;
Dept Pharmacol & Toxicol, Univ. Utah, Salt Lake City, UT
Abstract: We previously reported that the dopamine (DA)-related neuropeptides, neurotensin
and substance P, associated with limbic and extrapyramidal pathways, are affected by nicotine
administration through activation of nicotinic and D-2 DA receptors. Because metenkephalin
(Menk) systems are also closely related to both limbic and extrapyramidal DA projections, we
determined if nicotine treatment alters this neuropeptide. We focused primarily on the expression