[Abstract Title]. - Society for Neuroscience

the olfactory bulb and also directly to the striatum where they differentiate mainly into neurons
but also into glial cells. Using specific NPY receptor agonists, antagonist and KO mice, we
report that NPY-induced neuroproliferation is mediated by Y1 receptor subtype. Furthermore,
we found that Y1 receptor is highly expressed in the SVZ both at the mRNA and protein levels
and mainly by neuroblasts.
Conclusion: Stimulating endogenous SVZ neural stem cells by NPY may be of a potential
interest in cell replacement based therapies of neurodegenerative diseases affecting the striatum
such as Huntington‟s disease.
Disclosures: M. Decressac, None; L. Prestoz, None; J. Veran, None; A. Cantereau, None; M.
Jaber, None; A. Gaillard, None.
Poster
229. Neuronal and Glial Proliferation III
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 229.12/A12
Topic: A.02.a. Proliferation
Title: Differential post-lesional response of distinct populations of hippocampal precursors in
the young and aged brain
Authors: J. WALTER, S. KEINER, J. OBERLAND, A. KUNZE, O. WITTE, *C.
REDECKER;
Dept Neurol, Friedrich Schiller Univ., Jena D-07747, Germany
Abstract: During hippocampal neurogenesis slowly dividing precursor cells with astrocytic,
radial glia-like properties could be distinguished from highly dividing neuronal precursors in the
subgranular zone (SGZ). Several studies indicate that these distinct subtypes of precursors are
differentially stimulated under pathophysiological conditions. Even the relatively quiescent
radial glia-like precursors (type 1 cells) increase their proliferative activity within hours after
cortical infarcts. It is the aim of the present study, to analyze whether this proliferative response
is also present in the aged brain. To this purpose, we used the photothrombosis model to induce
focal infarcts in the forelimb cortex of 3 and 16 months old transgenic mice expressing greenfluorescent
protein (GFP) under control of the stem cell marker nestin. Sham-operated mice
served as controls. To label proliferating cells all mice received three single injections of
bromodeoxyuridine (BrdU, 50 mg/kg i.p. every 2 h) at day 4 after the infarct. Two hours after
the last injection the animals were transcardially perfused and processed for
immunocytochemistry using antibodies against BrdU, Nestin-GFP, glial fibrillary acidic protein
(GFAP) and doublecortin (DCX). Stereological analysis of BrdU-positive cells in the SGZ