[Abstract Title]. - Society for Neuroscience

Abstract: Forced swim produces a decrease in pain sensitivity in laboratory rodents, known as
swim stress-induced analgesia (SSIA). Pain perception and sensitivity to analgesics strongly
depend on genotype. Mice selectively bred for magnitude of SSIA display differential
emotionality in a variety of behavioral tests, different responses to SSRIs, and markedly
divergent morphine analgesia (so called ,,correlated traits”). These differences appear to be
determined by a number of unknown major genes. The present study examined gene expression
in brain structures in lines of mice bred for high (HA) and low (LA) SSIA. All mice were
experimentally naive (untreated). Expression profiling using microarrays allows for detailed
characterization of the gene networks that regulate animal‟s analgesia and the correlated traits.
To characterize individual differences in genetic correlates of SSIA and the correlated traits we
used 24 K oligo microarrays and transcriptome analyses were performed in hypothalamus,
hippocampus, raphe nucleus and frontal cortex. We found a different number of genes in each
brain structures differently expressed in HA and LA lines. These genes are involved in regulation
of biochemical pathways such as: gap junction, longterm potentiation, long-term depression,
axon guidance, neuroactive ligand-receptor interaction. Different activity of these pathways may
affect biological processes involved in SSIA, different emotionality in behavioral tasks of HA
and LA mice and different responses to desipramine, venlafaxine and s-citalopram. The results
suggest that selection for SSIA produces differential effects on transcriptome and activation of
biological processes in brain.
Disclosures: P. Lisowski, None; G. Juszczak, None; A.H. Swiergiel, None; J. Goscik,
None; M. Wieczorek, None.
Poster
270. Pain: Opioids and Other Analgesics II
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 270.4/HH3
Topic: D.08.r. Opioids and other analgesics
Support: NIDA Grant DA 19959 (to B.C.Y.)
Title: Tolerance following acute, intermittent and chronic treatment with oxycodone and
etorphine
Authors: *P. A. MADIA, S. V. DIGHE, S. SIROHI, B. C. YOBURN;
Col. of Pharm., St. John's Univ., Jamaica, NY
Abstract: Opioid analgesic treatment protocol may play a role in determining the magnitude of
tolerance. In this study, we evaluated the effect of treatment protocol on tolerance to morphine in