[Abstract Title]. - Society for Neuroscience

transmission electron microscopy (TEM) level.
EMFs have been used for years at the clinic as an adjunct in the treatment of diseases or in the
presence of ulcers well in bones, acting as a promoter of angiogenesis and this facilitates the
recovery of ulcers, bones or injured tissues with little blood circulation.
Male Wistar adult rats were maintained under controlled conditions with free access to food and
water. Three groups of 32 animals each were carefully handled according the Mexican Health
Laws and the National Institutes of Health (USA) Guidelines for the Care and Use of Laboratory
Animals.
In the experimental group (E) the animals were exposed to an electromagnetic field of 0.66mT
rms, a frequency of 120 Hz harmonic waves. The Helmholtz coils where the unanesthetized
animals were placed in a specially designed plastic cage. Exposure was for 2h daily for 7 days,
after 3 days habituation with no connection of the coils. In the sham exposed group (S) the
animals were treated similarly to those of the E group, but with no connection of the coils
throughout the experiments. In the control group (C) the animals were intact. All animals were
injected with 2 ml/Kg (v/w) colloidal carbon (CC) solution through the femoral vein and killed
by intraperitoneal injection of 50 mg/Kg (w/w) pentobarbital at 0, 1, 5 and 10 min after the CC
injection. The brain was fixed by immersion in 0.1M phosphate buffered-4% paraformaldehyde
solution. Circumventricular organs, sensori-motor cerebral cortex (SMCC) and hippocampus (H)
were dissected out to be embedded in epoxy resins. 0.5 κm-thick sections were stained with
toloudin blue to be examined under a light microscope equipped with a professional image
analysis system and 0.1κm-thick sections were stained with uranyl acetate-lead citrate to be
examined under TEM.
EMF-120Hz increased the CC-extravasation to parenchyma-intercellular space, also, cc was
observed in the intercellular unions and vesicles, which join to each other, forming a system of
pores or channels in endothelial cells, in fenestrae cells, in basal membrane, pericapillary space
and intercellular spaces of CVO, this probably due to morphological changes in capillaries or
another mechanism not even described.
Disclosures: Y.K. Gutierrez-Mercado, None; J. Bañuelos-Pineda, None; G. Serrano,
None; L. Cañedo-Dorantes, None; A. Feria-Velasco, None.
Poster
287. Blood Brain Barrier and CSF
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 287.8/RR2
Topic: C.07.b. Neurovascular unit
Title: Is there a relationship between neuronal and vascular protection in experimental
glaucoma?