[Abstract Title]. - Society for Neuroscience

Title: CaMKIIδ promotes axonal and dendritic development in hippocampal neurons
Authors: *V. A. GLODOWSKI, J. MCDERMOTT, C. FINK;
Biol. Sci., Univ. Wisconsin-Milwaukee, Milwaukee, WI
Abstract: CaMKII is a protein serine/threonine kinase that is the most abundant protein in the
hippocampus comprising up to 2% of hippocampal protein. There are four isoforms of CaMKII
which are found in mammals: α, β, γ and δ. The catalytic and regulatory domains are highly
homologous between the four isoforms. However, the association domain contains variable
inserts which affect the ability of CaMKII isoforms to interact with different substrates. Previous
studies have shown that CaMKII isoforms α and β play a role in the development of filopodia
and in spine formation. Furthermore, CaMKIIδ has been shown to regulate neuronal morphology
due to its ability to bind actin. This project explores whether CaMKIIδ, which binds to actin, also
plays a role in neuronal development, specifically in axonal and dendritic development. Using
cell cultures of hippocampal tissue taken from 1-2 day postnatal Sprague-Dawley rats and a
Dicer RNAi system, we reduced protein levels of the CaMKIIδ isoform. The length of the
primary axonal process, axonal branches and dendritic processes were significantly decreased in
the δ isoform. These results suggest that the CaMKIIδ isoform may play a role in neuronal
development.
Disclosures: V.A. Glodowski, None; J. McDermott, None; C. Fink, None.
Poster
231. Dendrite Growth and Branching: Signaling
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 231.19/B32
Topic: A.04.i. Dendritic growth and branching
Support: Grant-in-Aid for Scientific Research (B) #19300119 form the Ministry of Education,
Science, Sports and Technology of Japan
Science
Grant-in-Aid for JSPS Fellows #19-3007 from Japan Society for the Promotion of
Title: Developmental gene expression of the CaMK cascade in hippocampus and its roles in the
dendritic development of hippocampal neurons
Authors: *A. KAMATA 1,2 , K. FUKUNAGA 2 , H. KONDO 3 , H. SAKAGAMI 1 ;
1 Dept. of Anat., Kitasato Univ. Grad. Sch. of Med., Sagamihara, Japan; 2 Dept. of Pharmacol.,