[Abstract Title]. - Society for Neuroscience

1 Mol. Biomedicine, CINVESTAV-IPN, Distrito Federal, Mexico; 2 Kline Inst., New York, NY;
3 Insituto Nacional de Neurología y Neurocirugía Manuel Velazco Suárez, Distrito Federal,
Mexico
Abstract: Alzheimer Disease (AD) is characterized by hallmarks lesions: neuritic plaques and
neurofibrillary tangles (NFT). Progressive dementia is correlated with the appearance of NFT in
different regions of hippocampus and enthorinal cortex. These NFT are mainly composed by
filamentous aggregates of Tau protein as paired helical filaments (PHF). Previous studies
showed a proteolytic resistant fragment named as PHF core, containing a main portion of the Tau
microtubule binding domain, suggesting that this fragment is important for Tau abnormal
polymerization in AD. In the present study, we modified neural precursor cells primary cultures
(NPCs) by a retroviral system, in order to of evaluated the factor involve in the aggregates
generation of Tau. PHF core was used in two ways: 1) as a soluble fraction and 2) with a
membrane localization (IFNγR1-NMF), to elucidate the possible role of the membrane substrate
as a nucleation center for Tau polymerization. We observed that soluble PHF-core was able to
form a moderate quantity of β-sheet structures, but interestingly the presence of these structures
was higher in cells with IFNγR1-NMF expression. These data suggest that the interaction
between PHF core and plasma membrane could regulate the formation of abnormal fibrillary
deposits.
Disclosures: K. Lira-De Leon , None; M.A. De Anda-Hernandez, None; P. Figueroa-
Corona, None; M. Cardenas-Aguayo, None; V. Campos-Peña, None; M.A. Meraz-Rios,
None.
Poster
245. Tau and Alzheimer's disease
Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm
Program#/Poster#: 245.20/P2
Topic: C.01.h. Tau
Title: Expression of soluble and chimerical truncated tau (151-391) in neuronal cultures
Authors: *M. A. DE ANDA-HERNANDEZ 1 , K. I. LIRA-DE LEON 1 , P. FIGUEROA-
CORONA 1 , V. CAMPOS-PEÑA 2 , M. A. MERAZ-RIOS 1 ;
1 Mol. Biomedicine, CINVESTAV-IPN, Mexico, Mexico; 2 Inst. Nacional de Neurologia y
neurocirugia manuel velazco suarez, mexico, Mexico
Abstract: Alzheimer disease (AD) is a neurodegenerative progressive encephalic disorder,
characterized by behavioral changes associated to memory lost and disorientation. Is the most