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[Abstract Title]. - Society for Neuroscience

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immobilisation stress in 5-HTT-/- mice and their WT littermates (CD1 background). Moreover,<br />

sleep-wake cycles were monitored by polysomnographic recordings in parallel. Finally, the<br />

effects on sleep of pharmacological blockade of hcrt 1 receptor (SB-334867, i.p.) immediately<br />

be<strong>for</strong>e the stress session were analysed.<br />

Compared to WT mice, 5-HTT-/- mutants exhibited an enhanced hypocretinergic<br />

neurotransmission but reduced activation of serotonergic neurons (as evidenced by the number of<br />

double labelled c-Fos and Tryptophan hydroxylase 2 neurons) after immobilisation stress.<br />

Polysomnographic recordings showed that stress was followed by a delayed increase in REM<br />

sleep in WT mice but not in 5-HTT-/- mutants. Although acute blockade of hcrt-R1 by SB-<br />

334867 did not significantly affect vigilance states in either mouse strain under control<br />

conditions, this treatment restored the stress-induced augmentation of REM sleep in 5-HTT-/-<br />

mice.<br />

Altogether, the present data show that 5-HT plays a key role in REM sleep homeostasis after a<br />

stress challenge, and that hypocretinergic neurotransmission might underlie the disrupted sleep<br />

response to stress observed in mice with genetically-driven loss of the 5-HTT response. Finally,<br />

this study emphasizes the role of hypocretin/serotonin interactions in mediating normal sleep<br />

response to stress.<br />

Disclosures: A. Rachalski, None; C. Alexandre, None; M.B. Emerit , None; J. Bernard,<br />

None; M. Hamon, None; J. Adrien, None; V. Fabre, None.<br />

Poster<br />

285. Sleep: Molecular, Cellular and Pharmacology I<br />

Time: Sunday, November 16, 2008, 1:00 pm - 5:00 pm<br />

Program#/Poster#: 285.19/QQ35<br />

Topic: E.08.c. Sleep: Molecular, cellular, and pharmacology<br />

Support: USPHS grant NS20246<br />

USPHS grant RR20146<br />

<strong>Title</strong>: Cholinergic responses and intrinsic membrane properties of developing parafascicular<br />

neurons<br />

Authors: *M. YE, E. GARCIA-RILL;<br />

Neurobio., Univ. Arkansas Med. Sci., Little Rock, AR<br />

<strong>Abstract</strong>: Parafascicular neurons (Pf) receive cholinergic input from the pedunculopontine<br />

nucleus (PPN), which is active during waking and REM sleep. There is a developmental

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