TRADITIONAL POSTER - ismrm

1867. Assessment of Macrophage Depletion on Acute Cardiac Allograft Rejection by MRI
Danielle F. Eytan 1 , T Kevin Hitchens 1 , Qing Ye 1 , Yijen L. Wu 1 , Chien Ho 1
1 Pittsburgh NMR Center for Biomedical Research, Carnegie Mellon University, Pittsburgh, PA, United States
Poster Sessions
Abundant macrophage infiltration is observed in cardiac allograft rejection, yet their contribution to the rejection process and the tissue damage that results
remains unclear. Here we investigated the role these cells play in our rat model of acute cardiac rejection by selectively depleting circulating macrophages
using liposomal-clodronate. We used T2*-weighted imaging to detect immune-cell infiltration at sites of rejection by monitoring the accumulation of iron
oxide-labeled cells, and cardiac cine-tagging to detect regional myocardial function loss. Our results indicate that macrophages contribute to tissue damage
during acute rejection, and that their depletion may attenuate the damaging effects of rejection in rat cardiac allografts.
1868. In-Vivo Tracking of Single Phagocytic Cells in a Mouse Brain After Traumatic Brain Injury Using
Micron-Sized Iron-Oxide Particles
T. Kevin Hitchens 1,2 , Parker H. Mills 1,2 , Lesley M. Foley 1 , John A. Melick 3 , Patrick M. Kochanek 3,4 , Eric T.
Ahrens 1,2 , Chien Ho 1,2
1 Pittsburgh NMR Center for Biomedical Research, Carnegie Mellon University, Pittsburgh, PA, United States; 2 Department of
Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, United States; 3 Safar Center for Resuscitation Research, University
of Pittsburgh, Pittsburgh, PA, United States; 4 Department of Critical Care Medicine and Anesthesiology, University of Pittsburgh,
Pittsburgh, PA, United States
Cellular imaging is an important and growing field in magnetic resonance. The ability to non-invasively detect the trafficking and accumulation of cells in
vivo has broad implications for both a better understanding of biological processes and the development of novel treatments for numerous conditions. Here
explore using post processing techniques called Phase map cross-correlation Detection and Quantification or PDQ for detection and quantification of single
MPIO-labeled cells in vivo. PDQ uses phase information to calculate a magnetic dipole moment for each detected cell. This information can be used to
correlate labeled cell between serial scans and imaging methods.
1869. MR Imaging of Tumor Initiating Melanoma Cells
Sergey Magnitsky 1 , Alexander Roesch 2 , Stephen Pickup 1 , Meenhard Herlyn 2 , Jerry D. Glickson 1
1 Radiology, University of Pennsylvanian, Philadelphia, PA, United States; 2 Wistar Institute, Philadelphia, PA, United States
Melanoma cells were labeled with iron oxide particles and allowed to proliferate. Small iron-retaining sub-cell population with “original” iron concentration
has been detected after 21 days of proliferation. This sub-cell population exhibits high tumorigenicity, self-renewal capacity and drug resistance, and
therefore fulfills a “definition” of tumor initiating cells. After implantation of labeled cells into NOD/SCID mice, iron-retaining cells have been detected by
in vivo, ex vivo MRI and Prussian blue staining.
1870. MR Cell Tracking in Reperfused Myocardial Infarction with Microvascular Obstruction and
Haemorrhage: Fluorine-19 MR Could Be a Better Solution
Yuxiang Ye 1 , Thomas C. Basse-Luesekrink 1 , Paula Arias 2 , Kai Hu 2 , Thomas Kampf 1 , Vladimir Kocoski 3 ,
Xavier Helluy 1 , Peter M. Jakob 1,4 , Karl-Heinz Hiller 1,4 , Roland Jahns 2 , Wolfgang R. Bauer 2
1 Department for Experimental Physics 5, University of Wuerzburg, Wuerzburg, Bavaria, Germany; 2 Deptment of Internal Medicine I,
University Hospital Wuerzburg; 3 Institute for Virology & Immunobiology, University of Wuerzburg, Germany; 4 MRB Research
Center, Magnetic-Resonance-Bavaria, Wuerzburg, Germany
MR Cell tracking with iron oxide labeling has high sensitivity but could be severely interfered by strong local magnetic susceptibility effects. We show that
Fluorine-19 MRI could unambiguously detect blood monocytes/macrophages labeled with perfluorocarbon emulsion infiltrating the haemorrhagic
myocardial infarct (MI) core both in vivo and ex vivo in a rat model at 7-T, despite the presence of strong local magnetic susceptibility effects caused by
degraded hemoglobin products in microvascular obstruction or haemorrhage, which often occurs after reperfusion therapy . This finding suggests that
Fluorine-19 MRI could be a better approach for MR cell tracking in where local T2* effects interfere the detection of magnetically labeled cells.
1871. Migration of MPIO-Labeled Glioma Cells in the Rat Brain: Validation with Histology and
Fluorescence Microscopy
Divya Raman 1 , Anitha Priya Krishnan 2 , Scott Kennedy 3 , John Olschowka 4 , Sammy N'dive 2 , Delphine
Davis 5 , Walter G. O'Dell 2
1 Biomedical Engineering, University of Rochester, Rochester, NY, United States; 2 Radiation Oncology, University of Rochester,
Rochester, NY, United States; 3 Biophysics, University of Rochester, Rochester, NY, United States; 4 Neurobiology and Anatomy,
University of Rochester, Rochester, NY, United States; 5 Imaging Sciences, University of Rochester, Rochester, NY, United States
Our hypothesis is that paths of elevated diffusion provide a preferred route for migration of cancer cells away from primary tumor. This can be used to
improve radiation treatment of gliomas. Toward this end, we have developed a computational model of cell migration based upon MR-DTI to predict
microscopic spread of cancer in patients. Objective of this work is to track MPIO labeled rat glioma cells in rat brain and compare it to rat DTI model and
thereby demonstrate that tumor cells migrate farther from the site of engraftment along major fiber tracts compared to gray matter.
1872. SPIO-Labeled Natural Killer Cells: Cytotoxicity and in Vivo Imaging
Christiane L. Mallett 1,2 , Catherine Ramsay 1 , Paula J. Foster 1,2
1 Imaging Research Laboratories, Robarts Research Institute, London, Ontario, Canada; 2 Medical Biophysics, The University of
Western Ontario, London, Ontario, Canada
Purpose: We labeled natural killer cells and tested cytotoxicity against prostate cancer cells in vitro. In vivo MR tracking was performed. Methods: KHYG-1
cells were labeled with MoldayION by incubation. Toxicity against PC-3M prostate cancer cells was measured after 24 hours co-culture. Labeled KHYG-1