TRADITIONAL POSTER - ismrm

Poster Sessions
center, Amsterdam, Netherlands; 5 Pathology, VU University medical center, Amsterdam, Netherlands; 6 Epidemiology and
Biostatistics, VU University medical center, Amsterdam, Netherlands
Only few lesions in cortical gray matter (CGM) of multiple sclerosis (MS) patients can be visualized with conventional MRI. Quantitative MRI techniques
are more sensitive to cortical damage, but the histopathological correlates of quantitative MRI changes in the MS CGM are unclear. We aimed to define the
underlying pathology of cortical quantitative MRI changes, and to compare MRI visible and invisible lesions by histopathology. 16 brain slices from 10
chronic MS patients were imaged with qualitative and quantitative MRI at 1.5T. Regions of interests were correlated with histopathology.
Quantitative MRI measurements reflect the extent of cortical demyelination. Conspicuity of cortical GM lesions on conventional MRI is determined by
lesional size.
2073. Quantification of Formalin-Fixed MS Brain Tissue Parameters T1, T2*, PD and Phase at 7T and
Comparison with Histopathology
Cherian Renil Zachariah 1 , David Pitt 2 , Peter Wassenar 1 , Bradley D. Clymer 1 , Amir Abduljalil 1 , Michael V.
Knopp 1 , Petra Schmalbrock 1
1 Radiology, The Ohio State University, Columbus, OH, United States; 2 Neurology, The Ohio State University, Columbus, OH, United
States
Depiction of cortical demyelination in MS is still hampered by low contrast, spatial resolution and specificity.This study applies T2*-gradient echo and
inversion recovery turbo field echo (IR-TFE) sequences 7T to image formalin-fixed tissue specimen and measure T1,T2*,PD and phase differences. We
notice that PD maps and phase maps may be promising for enhancing cortical lesion depiction . Following MRI, specimen were cut and labeled with antimyelin
basic protein antibodies to detect myelin and with anti-CD68 antibodies to detect activated macrophages/microglia. Scanned histology slides were
scored for cortical lesions and compared to MRI
2074. MRI Texture Correlates of Pathological Findings in Post-Mortem Multiple Sclerosis Brain
Yunyan Zhang 1,2 , GR Wayne Moore 1 , Cornelia Laule 1 , Thorarin A. Bjarnason 2 , Piotr Kozlowski 1 , Alex L.
Mackay 1 , Anthony L. Traboulsee 1 , David K. B. Li 1
1 University of British Columbia, Vancouver, BC, Canada; 2 University of Calgary, Calgary, AB, Canada
Ten post-mortem brain samples from 3 MS subjects were imaged at 7T. Regions of interest were marked on histological sections staining for myelin and
axon, then were matched on MR images including lesions (14), normal appearing white matter (NAWM, 12) and regions of reduced myelin and axon
(rLrB). MRI texture analysis based on polar Stockwell Transform (PST) was performed. Texture was highest in lesions, intermediate in rLrB and lowest in
NAWM (p < 0.01) providing evidence that texture abnormality associates with tissue pathology. PST analysis may be a potential tool to quantify tissue
integrity in MS or other neurological disorders.
2075. Evaluating MACC for Improved MS Rater Agreement
David S. Wack 1 , Michael G. Dwyer 1 , Niels Bergsland 1 , Sara Hussein 1 , Robert Zivadinov 1
1 University at Buffalo, Buffalo Neuroimaging Analysis Center, Buffalo, NY, United States
The software method of Minimum Area Contour Change (MACC) is evaluated for use to improve same scan inter-rater agreement for the delineation of T2
hyper-intense MS lesions; and for the application of ROIs to follow up time points. The MACC method improves inter-rater agreement, and performs about
on par with another rater for the purpose of drawing lesion ROIs on a follow-up scan.
2076. Lesion Recognition in Multiple Sclerosis: A Sequence Comparison and Quantification Study at 3T
Tobias Kober 1,2 , Cristina Granziera 3,4 , Delphine Ribes 1,2 , Patrick Browaeys 5 , Myriam Schluep 6 , Katrin
Wohlfarth 7 , Reto Meuli 5 , Gunnar Krueger 2
1 Laboratory for functional and metabolic imaging, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland; 2 Advanced
Clinical Imaging Technology, Siemens Suisse SA - CIBM, Lausanne, Switzerland; 3 Department of Neurology, Hôpitaux
Universitaires de Genève, Lausanne, Switzerland; 4 Brain and Mind Institute, Ecole Polytechnique Fédérale de Lausanne, Lausanne,
Switzerland; 5 Department of Radiology, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland; 6 Department of Neurology,
Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland; 7 H IM MR PLM AW Neurology, Siemens AG, Erlangen, Germany
Detection and radiological characterisation of multiple sclerosis (MS) lesions is an essential part both of clinical diagnosis and MS research. Ten early-stage
MS patients and ten controls were included in this study aiming at (i) comparing five different high-resolution imaging sequences (FLAIR, MP-RAGE, DIR,
SPACE, MP2RAGE) and (ii) quantifying T1 relaxation times of lesions with respect to their location in the brain. Results suggest that the DIR sequence is
the most sensitive for total lesion count, followed by the MP2RAGE. Confirming previous studies, T1 relaxation times were found to be overall prolonged.
2077. Pre-Filling MS Lesions on T1 and T2-Weighted Images for Improved Tissue Segmentation
Jonathan S. Jackson 1,2 , Declan Chard 2 , Antonia Ceccarelli 1 , Elisa Dell'Oglio 1 , Ashish Arora 1 , Mohit
Neema 1 , Rohit Bakshi 1 , David Miller 2 , Claudia Angela Michela Wheeler-Kingshott 2
1 Laboratory for Neuroimaging Research, Brigham & Women's Hospital, Harvard Medical School, Brookline, MA, United States;
2 Department of Neuroinflammation, UCL Institute of Neurology, London, United Kingdom
Robust lesion in-painting has been demonstrated on T1 and T2-weighted images. Many automated algorithms rely on accurate histograms for segmentation;
therefore this approach to lesion WM correction based on the global histogram is appropriate and strongly recommended as a pre-processing step for MS
images.