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Poster Sessions<br />

2367. Metabolic Profiling to Characterise Brain Tissues from a New Animal Model of Neurodegeneration<br />

with Lewy Body Pathology<br />

Philippine Camilla Geiszler 1,2 , Lynn Bedford 3 , R John Mayer 3 , Dorothee P. Auer 1 , Clare A. Daykin 2<br />

1 Division of Academic Radiology, School of Clinical Sciences, University of Nottingham, Nottingham, Nottinghamshire, United<br />

Kingdom; 2 Division of Molecular and Cellular Sciences, School of Pharmacy, University of Nottingham, Nottingham,<br />

Nottinghamshire, United Kingdom; 3 School of Biomedical Sciences, University of Nottingham, Nottingham, Nottinghanshire, United<br />

Kingdom<br />

This NMR spectroscopy-based metabolic profiling pilot study was conducted to examine the ability to characterise early effects of neurodegeneration in<br />

ubiquitin proteasome-depleted mice. In specific brain areas, these animals develop pyknotic nuclei preceding Lewy body-like neuronal inclusions and<br />

extensive neuronal loss. Cortices and hippocampi were extracted at the pyknotic nuclei stage. Liquid-state spectra, recorded at 400MHz, showed significant<br />

metabolic alterations (N-acetylaspartate, taurine, choline) in both areas indicative of substantial neuronal cell remodelling before neuronal death. The<br />

investigation demonstrated clearly the ability of NMR-based metabolic profiling techniques to aid in the characterisation of early neurodegeneration.<br />

2368. Assessing Lysosomal Pathology Using Magnetic Resonance Imaging<br />

Yuan Mei 1 , Robia G. Pautler 2<br />

1 Department of Psychology, Rice University, Houston, TX, United States; 2 Department of Molecular Physiology and Biophysics,<br />

Baylor College of Medicine, Houston, TX, United States<br />

There are many neurodegenerative diseases that cause lysosomal pathology including Alzheimer’s and Sandhoff disease. In these disorders, cellular<br />

irregularities disrupt the lysosomal membrane and cause the organelle to lose its internal acidity. Using a convertible T1 contrast agent sensitive to acidity,<br />

we hypothesize that magnetic resonance imaging (MRI) can be used to detect lysosome membrane permeabilization and loss of acidity in mouse models<br />

with lysosomal pathology. If successful, this methodology can potentially be applied in vivo and used as a tool to improve current diagnostic methods for<br />

neurological disorders such as Alzheimer’s disease.<br />

2369. Tract-Based Spatial Statistics (TBSS) Analysis Reveals Novel Changes in Lateral Thalamic Nuclei of<br />

Kainic Acid Treated Rats - Comparison of DTI and Histology<br />

Alejandra Sierra 1 , Kimmo Lehtimäki 1,2 , Teemu Laitinen 1 , Lassi Rieppo 3,4 , Asla Pitkänen 1,5 , Olli Gröhn 1<br />

1 Department of Neurobiology, A.I. Virtanen for Molecular Sciences, University of Kuopio, Kuopio, Finland; 2 Cerebricon Ldt.,<br />

Kuopio, Finland; 3 Department of Physics, University of Kuopio, Kuopio, Finland; 4 Department of Anatomy, Institute of Biomedicine,<br />

University of Kuopio, Kuopio, Finland; 5 Department of Neurology, Kuopio University Hospital, Kuopio, Finland<br />

Diffusion tensor imaging (DTI) in combination with tract-based spatial statistics (TBSS) analysis provides valuable anatomical information about changes in<br />

brain areas contributing to epileptogenic process. Lateral thalamic nuclei are one of the areas highlighted in TBSS showing increased FA 6 months after<br />

status epilepticus in rats. The present work is focused to characterize the interrelationship of histopathological changes and ex vivo DTI in combination with<br />

TBSS analysis using several histological stainings and polarized light microscopy.<br />

2370. Brain Behavior Relationship in Wild-Type Mice and a Mouse Model of Huntington’s Disease<br />

Jurgen Germann 1 , Jeffrey B. Carroll 2 , Christine Laliberte 1 , R. M. Henkelman 1 , Michael R. Hayden 2 , Jason<br />

P. Lerch 1<br />

1 The Mouse Imaging Centre, The Hospital for Sick Children, Toronto, Ontario, Canada; 2 Centre for Molecular Medicine and<br />

Therapeutics, Child and Family Research Institute, University of British Columbia, Vancouver, British Columbia, Canada<br />

We examined brain-behavior correlations in mice using MRI and 4 behavioural tests: Rotarod, Forced-Swim, Pre-pulse-Inhibition and Open Field test.<br />

Secondly, we investigated how these relationships are altered in a Huntington’s disease (HD) mouse model. Strong correlations were found in the wild-type<br />

mice identifying functional networks related to motor function, stress and anxiety, cortical gating and memory. The correlations are an expression of learning<br />

induced structural changes and provide insight into the study of brain networks controlling behavior; their absence in the HD mice could provide some<br />

insight into disease processes as they interfere with the changes normally induced by learning.<br />

2371. Diffusion Kurtosis in a Symptomatic Rat Model of Huntington’s Disease: Selective Grey and White<br />

Matter Pathology<br />

Ines Blockx 1 , Marleen Verhoye 1 , Dirk Poot 2 , Johan Van Audekerke 1 , Huu Phuc Nguyen 3 , Stephan Von<br />

Hörsten 4 , Jan Sijbers 2 , Annemie Van der Linden 1<br />

1 Bio Imaging lab - University of Antwerp, Antwerp, Belgium; 2 Vision Lab - University of Antwerp, Antwerp, Belgium; 3 Department<br />

of Medical Genetics - University of Tübingen, Tübingen, Germany; 4 Experimental Therapy - Friedrich-Alexander University,<br />

Erlangen, Germany<br />

Diffusion Kurtosis Imaging (DKI) quantifies the well known non-gaussianity of the diffusion process in biological tissue and is therefore an indicator of<br />

microstructural complexity. HD is a progressive late-onset neurodegenerative disorder and is characterized by the formation of huntingtin aggregates and<br />

degeneration of the corticostriatal network. In the present study, we used the microstructural sensitivity of DKI, to detect neurodegeneration in symptomatic<br />

tgHD rats at the age of 16 months. Region of interest analyses revealed significant differences of DT and DK parameters in grey (caudate putamen) and even<br />

in white matter (external capsula) structures.<br />

2372. Areas of Susceptibility of the Predisposed Immature Rat Brain to Hyperthermic Seizures and<br />

Resultant Neurodevelopment Delay : An MRI and PET Study<br />

Olivier Clerk-Lamalice 1 , Pierre Gravel 2 , Luc Tremblay 1 , Roger Lecomte 1 , Lionel Carmant 3,4 , Martin<br />

Lepage 1<br />

1 Centre d’imagerie moléculaire de Sherbrooke, Université de Sherbrooke, Sherbrooke, Quebec, Canada; 2 Département de radiologie,<br />

Hôpital Notre-Dame du Centre Hospitalier de l'Université de Montréal, Montreal, Quebec, Canada; 3 Centre de recherche de l’hôpital

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