TRADITIONAL POSTER - ismrm

Poster Sessions
1038. In Vivo High-Resolution Magic Angle Spinning Proton MR Spectroscopy of Small Whole-Model
Organism
C. Elegans
Valeria Righi 1,2 , Alex A. Soukas 3 , Gary Ruvkun 3 , A Aria Tzika 1,2
1 NMR Surgical Laboratory, Department of Surgery, Massachusetts General Hospital and Shriners Burns Institute, Harvard Medical
School, Boston, MA, United States; 2 Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Athinoula
A. Martinos Center for Biomedical Imaging, Boston, MA, United States; 3 Department of Genetics, Massachusetts General Hospital,
Harvard Medical School, Boston, MA, United States
We demonstrate metabolic biomarker profiles with high-resolution magic angle spinning proton MR spectroscopy (HRMAS H1 MRS) of living
Caenorhabditis elegans (C. elegans) worms. This work opens up perspectives for the use of H1 HRMAS-MRS as a metabolic profiling method for C.
elegans. Because it is amenable to high throughput and is shown to be highly informative, this approach may lead to a functional and integrated metabolomic
analytic approach of the small organism C. elegans, which has been used extensively in studies of aberrant metabolism, and should help in identifying,
investigating, and even validating new pharmaceutical targets for metabolic diseases.
1039. Morphologic Abnormalities of Mucopolysaccharidosis Type VII Characterized by High Resolution
MRI in a Mouse Model
Ilya Michael Nasrallah 1 , Sungheon Kim 2 , Ranjit Ittyerah 1 , Stephen Pickup 1 , John H. Wolfe 3,4 , Harish
Poptani 1
1 Department of Radiology, University of Pennsylvania, Philadelphia, PA, United States; 2 New York University; 3 Departments of
Pathobiology and Pediatrics, University of Pennsylvania; 4 Children's Hospital of Philadelphia
Mucopolysaccharidosis Type VII (MPS VII) is one of the degenerative lysosomal storage diseases characterized by intracellular vacuolization. Using highresolution
MRI in a mouse model of MPS VII with manual segmentation, we identify decreases in corpus callosum and anterior commisure volume and
slight increase in hippocampus volume in mutant mice. A decrease in corpus callosum volume thickness is confirmed at histology. These parameters could
be used for monitoring experimental response to gene therapy treatments.
1040. MRI Phenotyping of Craniofacial Development in Transgenic Mice Embryos
Hargun Sohi 1 , Seth Ruffins 1 , Yang Chai 2 , Scott Fraser 1 , Russell Jacobs 3
1 Caltech; 2 USC; 3 Caltech, Pasadena, CA, United States
Microscopic MRI (μMRI) is an emerging technique for high-throughput phenotyping of transgenic mouse embryos, and is capable of visualizing
abnormalities in craniofacial development. μMRI methods rely on reduction of the tissue T1 relaxation time by penetration of a gadolinium chelate contrast
agent. The use of contrast agents is aimed at reducing the T1 relaxation time of the sample thus permitting a decrease in acquisition scan time, and/or
increase in image signal-to-noise ratio (SNR), and/or increase in spatial resolution. In this work we apply these technologies to delineating changes in a
murine cleft palate model system.
1041. Relaxivity Tissue Differentiation Among Gd-Based Contrast Agents in Ex-Vivo Mouse Embryo
Imaging
Michael David Wong 1 , X Josette Chen 1 , R Mark Henkelman 1
1 Mouse Imaging Centre, Hospital for Sick Children, Toronto, Ontario, Canada
The role of MRI in developmental biology, specifically in mouse embryo organogenesis and phenotyping, is significantly increasing due to technologies that
allow for high image resolution and throughput. The majority of ex-vivo MRI mouse embryo studies improve image contrast and SNR by immersing the
sample into some concentration of Gd-based contrast agent. It is widely believed that all gadolinium-based contrast agents have identical tissue interactions
and provide similar MRI images despite the differences in Gd-chelates. Here, relaxivity (r1) variation amongst mouse embryo organs is observed for one
class of contrast agents, while homogeneity is seen throughout the embryo for another.
1042. Contrast Enhancement in Preserved Zebra Finch Brains Utilizing Low Temperatures at High
Magnetic Fields
Parastou Foroutan 1,2 , Susanne L. T. Cappendijk 3 , Samuel C. Grant 1,2
1 Chemical & Biomedical Engineering, The Florida State University, Tallahassee, FL, United States; 2 CIMAR, The National High
Magnetic Field Laboratory, Tallahassee, FL, United States; 3 Biomedical Sciences, College of Medicine, The Florida State University,
Tallahassee, FL, United States
Temperature is evaluated as an easy method of increasing contrast in preserved tissue. In this study, excised, fixed brains from the adult male zebra finch
were scanned at multiple temperatures between 5-25 Celsius. Relaxation (T1, T2 and T2*), signal-to-noise, relative contrast and contrast-to-noise were
measured at each temperature. In addition, high-resolution 3D gradient recalled echo scans were acquired at 40-micron isotropic resolution at each
temperature. Although all relaxation mechanisms displayed decreases with temperature, only T2* contrast displayed structural enhancement. The
ramifications of these findings are discussed with respect to microimaging studies of preserved tissue samples.
1043. Phenotyping a Novel Mouse Model of Congenital Heart Disease Using μMRI
Jon Orlando Cleary 1,2 , Francesca C. Norris 3,4 , Karen McCue 5 , Anthony N. Price 3 , Sarah Beddow 5 , Roger
J. Ordidge 2,6 , Peter J. Scambler 5 , Mark F. Lythgoe 3
1 Centre for Advanced Biomedical Imaging, Department of Medicine and UCL Institute of Child Health , University College London,
London, United Kingdom; 2 Department of Medical Physics and Bioengineering, University College London, London, United
Kingdom; 3 Centre for Advanced Biomedical Imaging, Department of Medicine and UCL Institute of Child Health, University College
London, London, United Kingdom; 4 Centre for Mathematics and Physics in the Life Sciences and EXperimental Biology
(CoMPLEX), University College London, London, United Kingdom; 5 Molecular Medicine Unit, UCL Institute of Child Health,