TRADITIONAL POSTER - ismrm

Poster Sessions
MRA with 1.6mm slice thickness, diameters were most accurately demonstrated. In CE-MRA, diameters of the tube were significantly more accurate with
parallel imaging than with ZIP.
2267. Direct Visualization and Quantitation of CSF Flow in Shunts
Noam Alperin 1,2 , Sang H. Lee 1 , Leonardo Macedo 3 , Daniele RigamontI 4 , Ari Blitz 3
1 University of Miami, Miami, FL, United States; 2 Radiology, University of Miami, United States; 3 Radiology, Johns Hopkins,
Baltimore, MD; 4 Nuerosurgery, Johns Hopkins, Baltimore, MD
Diversion of excessive CSF from hydrocephalic brains by shunting is, in many cases, a life saving procedure. The down side of shunting is high failure rate.
Consequently, about 40% of the shunting procedures performed annually in the US are for shunt replacement. The decision for shunt replacement is
challenging as there is no reliable noninvasive test for shunt function. We present the first direct visualization and quantitation of CSF flow in ventricular
shunts using high temporal and spatial resolution cine phase contrast MR and automated flow quantitation technique. Volumetric flow rate through in patent
shunts were on the order of the CSF production rate.
Manganese - Enhanced MRI
Hall B Wednesday 13:30-15:30
2268. Using Manganese-Enhanced MRI (MEMRI) to Detect the Order of Neuronal Connections in the
Olfactory Pathway at the Level of Specific Layers
Der-Yow Chen 1 , Stephen J. Dodd, Daniel R. Glen, Ziad S. Saad, Alan P. Koretsky
1 NINDS, National Institutes of Health, Bethesda, MD, United States
MEMRI can be used for neuronal tracing in the brain. Here it is demonstrated that MEMRI identifies the laminar connections of the olfactory system and
traces layer-specific inputs to olfactory cortices. MnCl 2 was injected into nostrils or the olfactory bulb of rats, and they were imaged with this MRI technique
at several time points. The dynamic changes of Mn 2+ enhancement could be characterized by the arrival latency into each specific region. The olfactory
pathway from olfactory bulb to higher-order cortex was labeled in proper, known laminar order. Mn 2+ enhancement into the orbitofrontal cortex predicts that
connections from olfactory cortex innervate superficial layer of the orbitofrontal cortex. This is a connection that has not been previously mapped.
Therefore, MEMRI neural tracing is specific at the level of cortical layers in the olfactory pathway.
2269. Evaluation of the Applicability of Manganese-Enhanced and Dynamic Gadolinium-Enhanced Imaging
to Study the Role of Caveolin-1 in Blood-Retinal Barrier Integrity
Philippe Garteiser 1 , Bruce A. Berkowitz 2,3 , Debbie Saunders 1 , Rebecca Cranford 1 , Rheal A. Towner 1 ,
Michael H. Elliott 4
1 Advanced Magnetic Resonance Center, Oklahoma Medical Research Foundation, Oklahoma City, OK, United States; 2 Department of
Anatomy and Cell Biology, Wayne State University, Detroit, MI, United States; 3 Department of Ophthalmology, Wayne State
University, Detroit, MI, United States; 4 Department of Ophtalmology, Dean A McGee Eye Institute, University of Oklahoma Health
Sciences Center, Oklahoma City, OK, United States
Mn-enhanced MRI (MEMRI) has recently emerged as an important tool in retinal function studies. Caveolin-1 (Cav-1), the principal protein member of
caveolar membrane domains, is believed to be essential to blood-retinal barrier integrity and ion homeostasis of the retina. Here, we evaluate how MEMRI
and other MRI techniques may detect functional disruptions induced by cell type-specific knock out of the Cav-1 gene in mice. The MEMRI signature of
light and dark adaptation and the dynamic gadolinium-enhanced signal behavior of iodate-induced retinal impairments indicate that both methods have
sufficient sensitivity to warrant their application to cell-type specific Cav-1 ko mice.
2270. Layer-Specific Anatomical MRI of the Retina with Balanced Steady State Free Precession with and
Without Manganese Enhancement
Eric Raymond Muir 1,2 , Bryan H. De La Garza 2 , Sung-Hong Park 3 , Timothy Q. Duong 2
1 Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, United States; 2 Research Imaging Institute,
Ophthalmology/Radiology, UT Health Science Center San Antonio, San Antonio, TX, United States; 3 Research Imaging Institute,
Radiology, UT Health Science Center San Antonio, San Antonio, TX, United States
Anatomical MRI of the retina has previously reported 3-4 layers in the rodent retina using conventional gradient echo (GE) and spin echo MRI. Following
intraocular injection of manganese, seven layers were detected previously. This study explored balanced steady state free precession (bSSFP) MRI to image
the mouse retina at 35x35x200 µm. Moreover, we compared GE and bSSFP with and without intraocular manganese injection. We demonstrated that bSSFP
can reveal 7 layers without using contrast agent. Layers detected by bSSFP without manganese were consistent with those of manganese-enhanced MRI and
histology.
2271. Assessing in Vivo Axonal Transport Rates from Deep Brain Structures in Mouse Models of Human
Disease
Taeko Inoue 1 , Robia G. Pautler 1
1 Dept. of Molecular Physiology & Biophysics, Baylor College of Medicine, Houston, TX, United States
Axonal transport is an important cellular mechanism necessary for the normal function and viability of a neuron. As a result, deficits in axonal transport have
become associated with the development and progression of human diseases such as diabetes and neurodegenerative diseases such as Alzheimer’s disease.
Here we demonstrate the potential of Manganese Enhanced MRI (MEMRI) for measuring in vivo deficits of axonal transport in two mouse models of human
disease.