TRADITIONAL POSTER - ismrm

Poster Sessions
of HP 129Xe in showing sensitivity to alveolar microstructure changes in animals suggests that 129Xe, which is cheaper and more readily available, is also
suitable for ADC measurements. Here, we discuss 129Xe ADC imaging results from healthy volunteers and COPD patients with early stage emphysema and
show that 129Xe ADC imaging can successfully discriminate the two groups.
2546. Quantitative Prediction of Lung Disease with Hyperpolarized Gas MRI – Validation in a Murine
Model of Emphysema
Masaru Ishii 1,2 , Kiarash Emami 2 , John M. Woodburn 2 , Stephen J. Kadlecek 2 , Elaine Chia 2 , Jianliang Zhu 3 ,
Stephen Pickup 2 , Yi Xin 2 , Rahim R. Rizi 2
1 Department of Otolaryngology, Johns Hopkins University, Baltimore, MD, United States; 2 Department of Radiology, University of
Pennsylvania, Philadelphia, PA, United States; 3 Department of Surgery, University of Pennsylvania, Philadelphia, PA, United States
The sensitivity of two HP 3 He MRI-based measurements, gas diffusivity and ventilation, to elastase-induced changes in a murine model of emphysema is
studied in this work. The motivation is primarily the increasing interest in assessments of pulmonary disease models and assessments of therapeutic
interventions in transgenic murine disease models, which require that functional and structural lung imaging techniques be translated to a smaller scale. We
present a predictive model for calculating the probability that a section of lung originated from a diseased animal.
2547. Estimation of Rat Lung Surface to Volume Ratio and Xenon Diffusing Capacity Using Hyperpolarized
3 He and 129 Xe Gases
Matthew S. Fox 1,2 , Alexei Ouriadov 1 , William Dominguez-Viqueira 1,3 , Marcus Couch 1,2 , Giles E. Santyr 1,3
1 Imaging, Robarts Research Institute, London, Ontario, Canada; 2 Physics and Astronomy Dept, University of Western Ontario,
London, Ontario, Canada; 3 Medical Biophysics, University of Western Ontario, London, Ontario, Canada
Hyperpolarized 129 Xe is a novel gaseous contrast agent which also dissolves in the lung parenchyma and blood compartments, offering an interesting palette
of potential biomarkers of pulmonary disease. 129 Xe signals from the dissolved compartments have different chemical shifts and can be selectively saturated
and allowed to recovery as a function of delay time as in the chemically selective saturation recovery (CSSR) technique. We collected CSSR data and 3D
volumes from rat lungs in-vivo and explore both the Butler and Mansson model for estimations of surface to volume ratio, diffusing capacity and tissue
transit time.
2548. Anisotropic Nature of 3He Gas Diffusion in Mice Lungs
Emir Osmanagic 1 , Alexander L. Sukstanskii 2 , Mark S. Conradi 3 , James D. Quirk 2 , Dmitriy A. Yablonskiy 2
1 Electrical and Systems Engineering, Washington University, St. Louis, Misssouri, United States; 2 Radiology, Washington University,
St. Louis, Misssouri, United States; 3 Physics, Washington University, St. Louis, MO, United States
Diffusion-attenuated MR signal of 3He gas in lungs demonstrate non-mono-exponential dependence on b-value. It was previously suggested that such
behavior is a result of microscopically anisotropic but macroscopically isotropic nature of lung microstructure: diffusion in each airway is anisotropic, while
distribution of airway axes directions is isotropic. Hypothetically such non-mono-exponential dependence would also be present in a system of multiple
spherical compartments (mimicking alveoli) with a variety of sizes. Herein, we used experiments with three consecutive bipolar gradient pulses with
orthogonal and parallel gradient orientations to discriminate between such two systems. Our result confirmed microscopically anisotropic hypothesis.
2549. Quantitative Assessment of Lung Microstructure in Healthy Mice Using Lung Morphometry with
Hyperpolarized 3He Diffusion MRI
Emir Osmanagic 1,2 , Alexander L. Sukstanskii 3 , James D. Quirk 3 , Jason C. Woods 3,4 , Mark S. Conradi 4 ,
Dmitriy A. Yablonskiy 3,5
1 Electrical and Systems Engineering, Washington University, St. Louis, Misssouri, United States; 2 Radiology, Washington University,
St. Louis, MO, United States; 3 Radiology, Washington University, St. Louis, Misssouri, United States; 4 Physics, Washington
University, St. Louis, MO, United States; 5 Physics, Washington University, St. Louis, Misssouri, United States
Lung morphometry technique with hyperpolarized 3He allows quantification of lung geometrical parameters such as mean chord length Lm, surface-tovolume
ratio S/V and density of alveoli. It was demonstrated that in humans, it provides results similar to direct morphological measurements. Two
important modifications, however, are required to adopt this technique for studying lung microstructure in small animals – reduction in diffusion time and
modification of theoretical relationship between diffusion MR signal and lung microstructural parameters. Herein we provided such modifications and
demonstrated that measurements obtained with lung morphometry with hyperpolarized 3He MRI in mice are in agreement with literature data.
2550. The Effect of Locally Administered Glucocorticoid Budesonide on Ovalbumin Exposed Rats Assessed
by HP 3 He MRI
Jelena Pesic 1 , Frank Risse 1 , Simon Young 2 , Jim Britt 2 , Ignacio Rodriguez 3 , Lars E. Olsson 1
1 DECS Imaging and Antibodies, AstraZeneca R&D, Mölndal, Sweden; 2 Bioscience, AstraZeneca R&D, Charnwood, United
Kingdom; 3 Instituto de Estudios Biofuncionales, Universidad Complutense, Madrid, Spain
HP 3 He ADC imaging was used to assess the effect of a glucocorticoid budesonide on inflammation in ovalbumin challenged rats. Four groups of animals
were investigated: controls, vehicle treated, low and high dose budesonide treated. The ADC was significantly smaller in the vehicle group, indicating
reduced airspace in the alveoli, possibly due to plasma leakage into the alveoli. Treatment with budesonide decreased inflammation as shown by
significantly reduced eosinophil counts and higher ADC values than in the vehicle group.