TRADITIONAL POSTER - ismrm

Poster Sessions
3137. Quantitative and Local Mouse Brain Morphometry in Longitudinal MRI Studies
Alize Elske Hiltje Scheenstra 1 , Dana Suciu 2 , Michael Muskulus 3 , Melly S. Oitzl 4 , Johan H.C. Reiber 1 ,
Louise van der Weerd 5 , Jouke Dijkstra 1
1 Radiology, image processing, LUMC, Leiden, Netherlands; 2 Radiology, LUMC, Leiden, Netherlands; 3 Mathematical Department,
Leiden University, Leiden, Netherlands; 4 LACDR, Leiden University, Netherlands; 5 Department of Anatomy and Embryology,
LUMC, Leiden, Netherlands
We present the Moore-Rayleigh (MR) test as nonparametric statistical test for longitudinal brain MRI deformation based morphometry: A group of male
mice (n=10) was followed during exposure to the stress hormone hormone corticosterone for 2 weeks and a recovery period of 1 week. The results of the
MR test are comparable to volumetric based morphometry, but it enriches the analysis with its ability to detect also localized shape changes, which are still
significant under Bonferroni correction.
3138. Type I Errors in Whole Brain Voxel-Wise Analyses
David Matthew Carpenter 1 , Cheuk Ying Tang 1,2
1 Radiology, Mount Sinai School of Medicine, New York, United States; 2 Psychiatry, Mount Sinai School of Medicine, New York,
NY, United States
Voxel based analysis or Statistical Parametric Mapping (SPM) yields inconsistent results across studies. It is difficult to challenge the validity of SPM
results in published works and review submissions because the nature of the immense datasets that underlie VBA results prohibits its presentation in
journals. In this abstract we use a simple data set to explore sources of type I errors in areas that often yield positive results and present findings that can
serve as a guide for critiquing these SPM presentations.
3139. Propagation-Based Morphometry in an Ex Vivo Mouse Embryo Atlas – Assessment and Validation
Francesca C. Norris 1,2 , Jon O. Cleary 1,3 , Marc Modat 4 , Anthony N. Price 1 , Karen McCue 5 , Sarah Beddow 5 ,
Peter J. Scambler 5 , Sebastien Ourselin 4 , Mark F. Lythgoe 1
1 Centre for Advanced Biomedical Imaging, Department of Medicine and UCL Institute of Child Health, University College London,
London, United Kingdom; 2 Centre for Mathematics and Physics in the Life Sciences and EXperimental Biology (CoMPLEX),
University College London, London, United Kingdom; 3 Department of Medical Physics and Bioengineering, University College
London, London, England, United Kingdom; 4 Centre for Medical Image Computing, University College London, London, United
Kingdom; 5 Molecular Medicine Unit, UCL Institute of Child Health, University College London, London, United Kingdom
The increasing use of genetically modified mice has highlighted the need for effective phenotyping methods. Propagation-based morphometry (PBM) is an
emerging technique enabling non-invasive and rapid acquisition of volumetric data using an average population atlas for morphometric analysis. Thus, PBM
shows promise for combining high-throughput µMR imaging of late-gestation embryos with high-throughput analysis. We present the first study to assess
and validate the accuracy of volumes generated via PBM in an ex vivo mouse embryo atlas comprising three different groups. Preliminary results show
promise towards the broad applicability of this technique for phenotyping mutant mouse models.
3140. A New Approach to Mouse Brain Mapping
Marianne Dorothea Keller 1,2 , Charles Watson 3 , Kay Richards 4 , Rachel Buckley 5 , Nyoman Kurniawan 6 ,
Richard Beare 5 , Jana Vukovic 2 , Deming Wang 1 , Steven Yang 1 , Peter Zhao 7 , Nathan Faggian 4 , George
Paxinos 7 , Steven Petrou 4 , Gary Egan 4 , Perry Bartlett 2 , Graham Galloway 1 , David Reutens 8
1 Centre for Magnetic Resonance, University of Queensland, Brisbane, Qld, Australia; 2 Queensland Brain Institute, University of
Queensland, Brisbane, Qld, Australia; 3 Curtin University, Perth, WA, Australia; 4 Florey Neuroscience Institutes, Melbourne, Vic,
Australia; 5 Monash University, Melbourne, Vic, Australia; 6 Centre for Magnetic Resonance, University of Queensland, Brisbane,,
Qld, Australia; 7 Prince of Wales Medical Research Institute, Sydney, NSW, Australia; 8 Centre for Advanced Imaging, University of
Queensland, Brisbane, Qld, Australia
When working with mouse brain models it becomes apparent, that anatomically detailed, three dimensional atlases are not readily available. On one hand,
histological atlases are two dimensional, whereas three dimensional MRI atlases might only define 40 brain structures.
Our aim is to create an digital atlas using high resolution images produced by a 16.4 T MRI scanner, complemented by histological data. A higher grade of
segmentation, for example 45 structures in the cerebellum and 35 in the hippocampus, will enable the researcher to compare normal mouse brain anatomy to
pathological anatomical changes in models of disease.
3141. Developmental Changes in the Shape of Hippocampus in Children Aged from 6 to 9 Years Old
Muqing Lin 1 , Lutfi Tugan Muftuler 1 , Ke Nie 1 , Kevin Head 1 , Claudia Buss 2 , Elysia Poggi Davis 2 , Curt A.
Sandman 2 , Orhan Nalcioglu 1 , Min-Ying Lydia Su 1
1 Tu & Yuen Center for Functional Onco-Imaging, University of California, Irvine, Irvine, CA, United States; 2 Department of
Psychiatry and Human Behavior, University of California, Irvine, Irvine, CA, United States
The shape analysis of hippocampus was commonly applied to evaluate the progression of atrophy pattern in elderly patients, and in this study it was applied
to evaluate the changes in developmental brain in 48 children. The distance from the hippocampal surface to the central line was mapped to a 2D grid for
statistical analysis. The Permutation and t-test was applied to compare two age groups (6-7 vs. 8-9 y/o), and the regression analysis with age was also
performed. Significant differences were found in small areas of CA1 and subiculum; however, overall there is not a strong age dependence.