TRADITIONAL POSTER - ismrm

Poster Sessions
2626. Qualitative and Quantitative Evaluation of Time-Resolved Flow Analysis of Portal Venous
Hemodynamics of Liver Cirrhosis Patients and Volunteers
Zoran Stankovic 1 , Zoltan Csatari 1 , Peter Deibert 2 , Wulf Euringer 1 , Wolfgang Kreisel 2 , Susanne Eggerking 2 ,
Philipp Blanke 1 , Zahra Abdullah Zadeh 1 , Jürgen Hennig 1 , Mathias Langer 1 , Michael Markl 1
1 Department of Diagnostic Radiology and Medical Physics, University Hospital Freiburg, Freiburg, Baden Württemberg, Germany;
2 Gastroenterology, University Hospital Freiburg, Freiburg, Baden Württemberg, Germany
Time-resolved flow-sensitive 4D MRI permits qualitative and quantitative evaluation of portal venous hemodynamics of liver cirrhosis patients compared to
volunteers. Our results demonstrate a persistent reduction of the peak velocities in the portal venous system between patients and different age group
volunteers. Analysis showed significant correlation for peak and mean velocities between MRI and US measurements (r=0.53, p< 0.001) which served as
reference standard. In liver cirrhosis patients 3D MR velocity mapping may be a standardized technique for evaluating flow characteristics modification,
therapy monitoring or disease progression.
Metabolism, Diabetes & Spectroscopy
Hall B Tuesday 13:30-15:30
2627. Metabolic Profilings of Urine from High Fat-Fed Rats Based on 1 H NMR Metabolomics
Jingjing Xu 1 , Changqin Liu 2 , Shuhui Cai 1 , Jiyang Dong 1 , Zhong Chen 1
1 Department of Physics, Fujian Key Laboratory of Plasma and Magnetic Resonance, Xiamen University, Xiamen, Fujian, China; 2 The
First Hospital of Xiamen Affiliated to the Xiamen University, China
The biochemical variations of urine from chow- and high fat-fed rats were investigated using NMR-based metabolomics. Two groups can be discriminated
clearly according to the scores plot of partial least squares discriminant analysis. The plot of variable importance in projection shows that taurine, succinate,
hippurate, choline, citrate, dimethylamine, acetate, dimethylglycine, creatine, creatinine, tyrosine, glycine and lactate are contributed to the classification. In
addition, the metabolic change with the development of obesity was also explored.
2628. Effects of a High-Fat Diet on Multiple Organ Systems in Mice: A MRI and MRS Study
Sabrina Doblas 1 , Philippe Garteiser 1 , Joanna DeMoe 1 , Tim Griffin 1 , Luke Szweda 1 , Rheal Towner 1
1 Oklahoma Medical Research Foundation, Oklahoma City, OK, United States
Magnetic resonance imaging is an in vivo imaging technique well adapted to measure and localize body fat contents and study obesity. Twenty-week old
mice fed a high-fat or a normal diet were assessed on a 7 Tesla MRI system. Whole body, cardiac and knee joint images as well as cardiac 1H spectra were
obtained and processed to assess the effects of a high-fat diet on adipose tissue distribution, joint damage and cardiac function. Fat mice had larger hearts,
larger knee fat pads and fatter cardiac tissue than the lean animals.
2629. An Animal Model for the Study of Developmental Origins of Adult Disease Associated to Dietary Fetal
Fatty Acids: MRI Assessment
Kenneth Hollander 1 , Catherine Tempel-Brami 2 , Fred M. Konikoff 1 , Menahem Fainaru 3 , Alicia Leikin-
Frenkel 1
1 Minerva Center for Lipid Metabolism in the Liver, Sackler School of Medicine, Tel Aviv University, Israel; 2 Alfredo Federico
Strauss Center for Computational Neuro-Imaging, Tel Aviv University, Tel Aviv, Israel; 3 Department of Physiology, Sackler School
of Medicine, Tel Aviv University, Tel Aviv, Israel
The mammalian fetus is completely dependent on the fatty acids supplied by its mother inside the uterus. In the present study we analyzed the impact of fatty
acids in pregnant mother's isocaloric diet on obesity and insulin resistance in adult offspring (DOAD). Body fat in pregnant mothers and offspring was
measured by MRI and correlated with tissues fat and insulin resistance in adult offspring. Essential Fatty Acids prevented adult offspring obesity and insulin
resistance whereas saturated fatty acids promoted it. MRI measured body fat correlated with HOMA index, tissues lipid content and SCD activity in white
adipose tissue.
2630. AGAT-/- Mice: A Metabolic Puzzle of Energy Deficiency and Insulin Sensitivity
Patricia Maria Nunes 1 , Christine I. H. C. Nabuurs 1 , Dirk Isbrandt 2 , Cees Tack 3 , Arend Heerschap 1
1 Department of Radiology, Radboud University Nijmegen Medical Centre, Nijmegen, Netherlands; 2 Centre for Molecular
Neurobiology, Institute for Signal Transduction, Hamburg, Germany; 3 Department of General Internal Medicine, Radboud University
Nijmegen Medical Centre, Nijmegen, Netherlands
Whole body creatine depletion causes several disarrangements in brain and muscle. In these conditions, AGAT-/-mice, a mouse model for deficient creatine
biosynthesis, have enhanced food intake and permanent lower body weight which may reflect a higher substrate catabolism. We assessed ex vivo hepatic
triglyceride concentration and the respective synthesis contributions from de novo lipogenesis (DNL) and dietary free fatty acids, by 1H / 2H-NMR.
Additionally, we evaluated whole body glucose and insulin levels during a glucose tolerance test. Our results showed that AGAT-/- had lower hepatic
triglycerides and the contribution from DNL, to this pool, was increased. On the contrary, dietary fatty acids contribute less to the hepatic triglyceride pool.
This suggests that dietary fatty acids are preferentially recruited to high energy demanding tissues as muscle. These data matched with lower glucose and
insulin concentrations during the glucose tolerance test, reflecting an insulin sensitive phenotype.