TRADITIONAL POSTER - ismrm

Poster Sessions
2690. In Vivo Prediction of Spermatogenesis in Seminiferous Tubules Using High-Resolution Magnetic
Resonance Imaging and Machine-Learning Techniques in Combination
Masayuki Yamaguchi 1 , Natsumaro Kutsuna 2,3 , Ryutaro Nakagami 1,4 , Akira Nabetani 5 , Atsushi Nozaki 5 ,
Mamoru Niitsu 4 , Seiichiro Hasezawa 2,3 , Hirofumi Fujii 1,3
1 Functional Imaging Division, National Cancer Center Hospital East, Kashiwa, Chiba, Japan; 2 Graduate School of Frontier Sciences,
University of Tokyo, Kashiwa, Chiba, Japan; 3 Institute for Bioinformatics Research and Development-Japan Science and Technology
Agency, Chiyoda, Tokyo, Japan; 4 Graduate School of Human Health Sciences, Tokyo Metropolitan University, Arakawa, Tokyo,
Japan; 5 GE Healthcare Japan, Hino, Tokyo, Japan
Seminiferous tubules are stratified epithelia composed of germ cells and Sertoli cells. They produce sperm and normally are 200E00μm in diameter. We
have succeeded in visualizing rat seminiferous tubules on in vivo MRI using a 3T scanner. In addition, the machine-learning technique allowed automatic
classification of testicular regions on MRI into normal and abnormal spermatogenesis in chemotherapy-induced injury in rat testes. If these techniques are
implemented in clinics in the future, they will be a helpful tool in reproductive medicine for infertile males.
2691. A Reference Region Tracer Distribution Model Analysis of Rat Penile Vascular Changes by DCE.
H. Carl Le 1 , Nelson Bennett 2 , Raanan Tal 2 , Dov Winkleman 1 , John Mulhall 3 , Jason Koutcher 1,4
1 Medical Physics, MSKCC, New York, NY, United States; 2 Urology, MSKCC, New York, NY, United States; 3 Surgery, MSKCC,
New York, NY, United States; 4 Medicine, MSKCC, New York, NY, United States
Sildenafil is effective in restoring penile blood flow in alleviating erectile function, a common side effect from radical prostatectomy. We have used DCE
MRI to image rat corpora cavernosum post nerve injury with and without sildenafil treatment. The effect of sildenafil on the corpora cavernosal vascular
volume changes are detected and can be used to monitor penile vascular health in clinic.
2692. Quality of Proton Magnetic Resonance Spectroscopic Imaging (MRSI) with and Without an
Endorectal Coil: A Phantom Study
Jian Wang 1 , Jian-ping Lu 1 , Tom W J Scheenen 2
1 Radiology, Changhai Hospital, Shanghai, China; 2 Radiology, Radboud University Medical Centre Nijmegen, Nijmegen, Gelderland,
Netherlands
The quality of data acquisition and post-processing of proton MRSI with and without an endorectal coil at 1.5T and 3.0T was assessed with the use of a
prostate phantom. With fixed spine and body array surface coils and an endorectal coil, 3D MRSI was performed repeatedly with 1) all coils, 2) only
endorectal coil and 3) only surface coils. The choline + creatine/citrate (CC/C) ratio of each voxel was semi-automatically calculated and compared between
different coil use and field strengths. Significant differences in CC/C existed between different field strengths and different locations within the phantom,
when these locations had large differences in magnetic field homogeneity.
2693. Proton and Sodium MR Imaging of in Vivo Human Prostate Using Dual-Tuned Body and Endorectal
Coils at 7T
Kyongtae Ty Bae 1 , Jung-Hwan Kim 1 , Alessandro Furlan 1 , Chan Hong Moon 1 , Bumwoo Park 1 , Tiejun
Zhao 2
1 University of Pittsburgh, Pittsburgh, PA, United States; 2 MR Research Support, Siemens Healthcare, Pittsburgh, PA, United States
We have demonstrated the feasibility of 1H and 23Na imaging of in vivo human prostate using dual-tuned body surface Tx/Rx and endorectal Rx only coils.
Our imaging technique was tested on normal human volunteers. Further improvement of this technique may facilitate the diagnosis of prostate cancer.
2694. Quantitative MRI Assessment of Matrix Development in Cell-Seeded Natural Urinary Bladder Smooth
Muscle Tissue-Engineered Constructs
Hai-Ling Margaret Cheng 1,2 , Syed S. Islam 3 , Yasir Loai 3 , Roula Antoon 3 , Marine Beaumont 1 , Walid A.
Farhat 3
1 Research Institute & Diagnostic Imaging, The Hospital for Sick Children, Toronto, Ontario, Canada; 2 Medical Biophysics, University
of Toronto, Toronto, Ontario, Canada; 3 Division of Urology, The Hospital for Sick Children, Toronto, Ontario, Canada
Cell-seeded natural tissue scaffolds hold promise for tissue-engineering large organs (e.g. the urinary bladder matrix for regenerating different tissue types).
However, our understanding of cell-natural matrix interaction is limited, and its influence on MRI characterization is unknown. This study explores
quantitative MRI at 1.5 T for investigating cell-matrix interaction and matrix development in a smooth muscle cell-seeded bladder model. Competing with
cell presence was matrix degradation due to cell-released collagenase, noted for the first time and perhaps unique to natural matrices. Quantitative T1, T2,
and diffusion measurements are consistent with collagen breakdown, with multicomponent T2 providing the best specificity.
2695. The Acellular Matrix for Bladder Tissue-Engineering: A Quantitative MRI Study
Hai-Ling Margaret Cheng 1,2 , Yasir Loai 3 , Marine Beaumont 1 , Walid A. Farhat 3
1 Research Institute & Diagnostic Imaging, The Hospital for Sick Children, Toronto, Ontario, Canada; 2 Medical Biophysics, University
of Toronto, Toronto, Ontario, Canada; 3 Division of Urology, The Hospital for Sick Children, Toronto, Ontario, Canada
Scaffolds derived from natural tissue acellular matrix (ACM) possess native biomechanical and biological properties difficult to achieve with synthetic
materials. Despite their promise, ACM optimization is needed and remains in early development. This study investigates the bladder ACM, which is useful
for regenerating various tissues, and effects of incorporating hyaluronic acid (HA), a natural biomaterial useful in tissue regeneration. Quantitative MRI
measurements (T1, T2, diffusion) at 1.5 Tesla are consistent with HA presence and two-fold water uptake from HA incorporation, with multicomponent T2
distinguishing the two effects. These results provide baseline MRI data for studying further manipulation such as cell-seeding.