TRADITIONAL POSTER - ismrm

Poster Sessions
University College London, London, United Kingdom; 6 Wellcome Trust Advanced MRI Laboratory, University College London,
London, United Kingdom
CHARGE and DiGeorge syndromes are conditions associated with haploinsufficiency of specific genes (CHD7 and TBX1) and are characterised by
cardiovascular defects. Knockout mice are an important tool in genetic studies, allowing genes implicated in congenital defects to be identified and
characterised. Micro-MRI is an emerging technique for high-resolution cardiac phenotyping, enabling the acquisition of 3D images of multiple embryo in a
single scan. Given the phenotypic overlap of these conditions, we examined heart morphology in novel double-knockout mouse embryos (Chd7+/-Tbx1+/-),
performing an assessment using MRI. In particular, we identified an increased incidence of ventricular septal defects in these mice.
1044. Optimised µMRI for Phenotyping the Tc1 Model of Down Syndrome
Jon Orlando Cleary* 1,2 , Francesca C. Norris* 3,4 , Frances K. Wiseman 5 , Anthony N. Price 3 , ManKin Choy 3 ,
Victor L.J. Tybulewicz 6 , Roger J. Ordidge 2,7 , Elizabeth M.C. Fisher 5 , Mark F. Lythgoe 3
1 Centre for Advanced Biomedical Imaging, Department of Medicine and UCL Institute of Child Health , University College London,
London, United Kingdom; 2 Department of Medical Physics and Bioengineering, University College London, London, United
Kingdom; 3 Centre for Advanced Biomedical Imaging, Department of Medicine and UCL Institute of Child Health, University College
London, London, United Kingdom; 4 Centre for Mathematics and Physics in the Life Sciences and EXperimental Biology
(CoMPLEX), University College London, London, United Kingdom; 5 Department of Neurodegenerative Disease, UCL Institute of
Neurology, University College London, London, United Kingdom; 6 MRC National Insitiute for Health Research, London, United
Kingdom; 7 Wellcome Trust Advanced MRI Laboratory, University College London, London, *equal contribution
‘Staining’ brain tissue with MR contrast agents is a key part of MR microscopy, enabling enhanced delineation of structures. Although excised brains allow
agent to quickly penetrate into tissue, brains left in-skull are less susceptible to damage during tissue extraction and imaging, resulting in more accurate
morphometric analyses. We sought to develop an optimised preparation and scanning protocol for imaging adult mouse brains in-skull, determining the
timecourse for agent to penetrate into intact brain. Using this protocol we assessed phenotype in Tc1 mice – a model of Down Syndrome. We identified
ventricular enlargement in 10 of 14 transgenic Tc1+ mice imaged.
1045. MR Microscopy of Zebrafish
Miriam Scadeng 1 , Ellen Breen 2 , Nathan Gray 1 , David Dubowitz 1
1 Radiology, UC San Diego, San Diego, CA, United States; 2 Medicine, UC San Diego, San Diego, CA, United States
Being a relatively new animal model there is no comprehensive 3D anatomical atlas onto which temporal or spatial data can be projected. In addition,
methods for in-vivo imaging of adult fish are needed if zebrafish researchers are to benefit from functional MR imaging techniques such as MRS, MEMRI,
BOLD and even ASL that are routinely being used in mouse models of disease. The major challenges include the very small size of the fish, and imaging the
live fish in water. We present methods for in-vivo MRI of zebrafish, and a 3D atlas of zebrafish anatomy.
1046. Visualization of Vascular Casts Using 3D MR Imaging
Ian Rowland 1 , Joseph Heintz 2 , Douglas Steeber 3 , Ralph Albrecht 2
1 Department of Radiology, University of Wisconsin, Madison, WI, United States; 2 Department of Animal Sciences, University of
Wisconsin, Madison, WI, United States; 3 Department of Biological Sciences, University of Wisconsin, Milwaukee, WI, United States
This study demonstrates that at 4.7T, using a standard 3D gradient echo sequence, images of vascular casts prepared using established corrosion casting
techniques may be obtained with an isotropic resolution