TRADITIONAL POSTER - ismrm

Poster Sessions
2631. In Vivo High-Resolution Magic Angle Spinning Proton MR Spectroscopy of Drosophila Melanogaster
Flies as a Model System to Investigate Obesity
Valeria Righi 1,2 , Yiorgos Apidianakis 3 , Dionyssios Mintzopoulos 1,2 , Loukas G. Astrakas, 1,4 , Laurence G.
Rahme 3 , A Aria Tzika 1,2
1 NMR Surgical Laboratory, Department of Surgery, Massachusetts General Hospital and Shriners Burns Institute, Harvard Medical
School, Boston, MA, United States; 2 Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Athinoula
A. Martinos Center for Biomedical Imaging, Boston, MA, United States; 3 Molecular Surgery Laboratory, Department of Surgery,
Massachusetts General Hospital and Shriners Burns Institute, Harvard Medical School, Boston, MA, United States; 4 Department of
Medical Physics, University of Ioannina, Ioannina, Greece
We demonstrate biomarker profiles with high-resolution magic angle spinning proton MR spectroscopy (HRMAS H1 MRS) of live Drosophila melanogaster
flies. We show that the metabolic HRMAS MRS profiles of adipokinetic hormone receptor (akhrnull) mutant flies, which have an obesity phenotype, are
different from isogenic control strain flies (akhrrev). Our approach advances the development of novel, in vivo, non-destructive research approaches in
Drosophila, suggests biomarkers for investigation of biomedical paradigms, and thus may contribute to novel therapeutic development in obesity.
2632. Quantification of Adipose Tissue Depots in the Obese Thigh During Weight Loss Using Dixon Method
Curtis L. Johnson 1 , Mina C. Mojtahedi 2 , Diego Hernando 3,4 , Dimitrios C. Karampinos 1,4 , Matthew P.
Thorpe 2 , Danchin Chen 1 , Ellen M. Evans 2,5 , John G. Georgiadis 1,4
1 Mechanical Science and Engineering Department, University of Illinois at Urbana-Champaign, Urbana, IL, United States; 2 Division
of Nutritional Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, United States; 3 Electrical and Computer Engineering
Department, University of Illinois at Urbana-Champaign, Urbana, IL, United States; 4 Beckman Institute for Advanced Science and
Technology, University of Illinois at Urbana-Champaign, Urbana, IL, United States; 5 Department of Kinesiology and Community
Health, University of Illinois at Urbana-Champaign, Urbana, IL, United States
MRI was used before and after a weight loss intervention investigating the effect of diet on body composition in obese, older women to quantify changes in
adiposity in the thigh. A two-point Dixon method was used to separate fat and water images in order to quantify subcutaneous, intermuscular, and
intramuscular fat as well as muscle in the thigh before and after weight loss for two groups of subjects, one taking a protein supplement and the other taking
a carbohydrate for control. Results showed greater loss of adipose tissue and retention of muscle for the protein group compared to the control.
2633. Magnetic Resonance Imaging of the Pancreatic Vasculature in Type 1 Diabetes
Zdravka Medarova 1 , Zeynep Onder 1 , Marytheresa Ifediba 1 , Dale Greiner 2 , Guangping Dai 1 , Gerrardo
Castillo 3 , Elijah Bolotin 3 , Anna Moore 1
1 Molecular Imaging Lab, Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General
Hospital, Charlestown, MA, United States; 2 Department of Medicine, University of Massachusetts Medical School, Worcester, MA,
United States; 3 PharmaIN, Ltd, Seattle, WA, United States
Vascular changes are commonly associated with many pathologies, including, cancer, arthritis, and diabetes. In type 1 diabetes, autoimmune lymphocytic
infiltration progresses over many years, culminating in the destruction of a critical mass of insulin-producing beta-cells, and ultimately, in hyperglycemia
and metabolic dysregulation. Vascular parameters, such as vascular volume, flow, and permeability are an important disease biomarker. It is important to
monitor the dynamics of pancreatic microvasculature noninvasively. Here, we describe the application of the long-circulating, paramagnetic T1 contrast
agent, PGC-GdDTPA-F for the noninvasive evaluation of vascular changes in a rat model of type 1 diabetes.
2634. No Relation Between Altered Oxidative Mitochondrial Function and Impaired Muscle Perfusion in
Type 2 Diabetes
Sandrine Duteil 1,2 , Sabrina Chiheb 3 , Claire Wary 1,2 , Emmanuel Cosson 3 , Aurélien Monnet 1,2 , Paul Elie
Valensi 3 , Didier Mesengeau 3 , Pierre Georges Carlier 1,4
1 NMR Laboratory, Institute of Myology, F-75651 Paris, France; 2 CEA, I²BM, MIRCen, IdM NMR Laboratory,, F-75651 Paris,
France; 3 Endocrinology, Jean Verdier Hospital, F- 93140 Bondy, France; 4 CEA, I²BM, MIRCen, IdM NMR Laboratory, , F-75651
Paris, France
Microangiopathic complications are a major concern in diabetes mellitus type II. Oxidative phosphorylation may also be impaired, with a yet imprecise
relationship to microangiopathy . An integrative investigation of metabolic and vascular response to stress was carried out to determine possible alterations
of perfusion and oxidative metabolism in calf muscle of 96 patients, categorized according to incidence of microangiopathy. Combining perfusion,
oxygenation and energetic measurements, we could show that mitochondrial activity was altered in patients with poorly controlled glycaemia, but unrelated
to reduced perfusion, which was common to all patients, while possible anomalies of oxygen diffusion might reflect diabetic microangiopathy.
2635. Imaging Pancreatic Islets Ex Vivo by Ultra High Field of 14T, Combining Manganese and Iron-Oxide
Enhanced MRI
Riikka J. Immonen 1 , Smaragda Lamprianou 2 , Laurent Vinet 2 , Paolo Meda 2 , Rolf Gruetter 1,3
1 Laboratory for functional and metabolic imaging, Ecole Polytechnique Fédérale de Lausanne, Lausanne, CH-1015, Switzerland;
2 Department of Cell Physiology and Metabolism, University of Geneva, Geneva, CH-1210, Switzerland; 3 Department of Radiology,
University of Geneva and Lausanne, Geneva and Lausanne, CH-1210 and CH-1015, Switzerland
In diabetes the gradual loss of pancreatic β–cells leads to impaired regulation of blood glucose levels. β–cell islets, 30-600μm in diameter, are sparsely
located accross the pancreas. We utilized for the first time ultra high field of 14.1T in combination of manganese- and iron-oxide nanoparticle-enhanced
MRI to assess pancreatic structures ex vivo. We were able to distinguish all the main pancreatic structures, including lobules and branching duct tree with
terminal acini. The manganese with glucose stimulus, without and together with the infusion of iron oxide particles, also delineated structures which are
likely to correspond to individual pancreatic islets.