TRADITIONAL POSTER - ismrm

Poster Sessions
2378. Effects of Continuously High Levels of Corticosteroids on Mouse Hippocampus – a Longitudinal in
Vivo MRI Study
Dana Suciu 1 , Alize E. H. Scheenstra 2 , Jouke Dijkstra 2 , Melly Sylvana Oitzl 3 , Louise van der Weerd, 1,4
1 Radiology, LUMC, Leiden, Netherlands; 2 Radiology - Image processing, LUMC, Leiden, Netherlands; 3 Medical Pharmacology,
LACDR, Leiden, Netherlands; 4 Anatomy, LUMC, Leiden, Netherlands
We report a longitudinal MRI investigation on mice chronically exposed to stress hormones (hypercorticism) to investigate hippocampal morphology. The
mice were implanted with a continuous corticosterone-releasing pellet (n=10) or a placebo cholesterol pellet (n=10). T2W MRI scans of the mouse brain
were taken over several weeks. Volumetric analysis by manual delineation using SPSS analysis and quantitative group-wise comparison using deformation
fields and a 3D Moore-Rayleigh test with Bonferroni correction were employed. Our study demonstrated that chronic hypercorticism in mice indeed leads to
volume loss in the hippocampus, which is at least partially reversible after recovery.
2379. Combined Vegf and Angiopoietin-1 Gene Transfer Using Aav Vectors After Spinal Cord Injury
Juan Jose Herrera 1 , Ponnada A. Narayana 1
1 Diagnostic and Interventional Imaging, The University of Texas Health Science Center at Houston, Houston, TX, United States
A consequence of spinal cord injury is the disruption of spinal vasculature, and it is this disruption that contributes to the initiation of cascade of biochemical
events leading to secondary damage from the ischemic and inflammatory responses Using adeno-associated viral vectors engineered to express Ang-1 and or
VEGF may stimulate angiogenesis and vessel maturation after spinal injury. Our study indicates that the syngeristic effect of both agents reduces spinal
vascular permeability and lesion volume determined by MRI leading to functional recovery.
2380. Characterization of Inner Ear Inflammation in Rodents Using in Vivo Dynamic Contrast Enhanced
Magnetic Resonance Imaging and Ex Vivo Light Microscopy
Johann Le Floc'h 1 , Beau Pontré 2 , Winston Tan 1 , Srdjan M. Vlajkovic 1 , Peter R. Thorne 1
1 Physiology, The University of Auckland, Auckland, New Zealand; 2 Centre for Advanced MRI, New Zealand
Inner ear inflammation is thought to be a major contributor to the development of hearing loss and balance disorders. We report the results of the in vivo
characterization of cochlear tissues inflammation induced by noise exposure or injection of bacterial lipopolysaccharide in two rodent species. The
anaesthetized animals were scanned using a 4.7T MRI system. The calculated signal enhancement due to the observed uptake of a contrast agent was greater
on inflamed than normal cochleae. MR findings correlated well with immunohistochemistry. We suggest that increase in gadodiamide uptake occurred as a
consequence of increased vascular permeability.
2381. Cerebral Metabolite Assessment in Low and High Capacity Running Rats Using 1H-MRS
Steven R. Roys 1,2 , Anjaneyulu Murugundla 3 , Su Xu 1 , Aurora Anderson 3 , Jiachen Zhuo 1 , Mark Limsam 3 , J
Choi 3 , Lauren Koch 4 , Steve Britton 4 , Krish Chandrasekaran 3 , Paul Yarowsky 5 , James Russell 3 , Rao P.
Gullapalli 1,2
1 Diagnostic Radiology & Nuclear Medicine, University of Maryland School of Medicine, Baltimore, MD, United States; 2 Core for
Translational Research in Imaging @ Maryland (C-TRIM); 3 Neurology, University of Maryland School of Medicine, Baltimore, MD,
United States; 4 Anesthesiology, University of Michigan, Ann Arbor, MI; 5 Experimental Therapeutics and Pharmacology, University
of Maryland School of Medicine, Baltimore, MD, United States
Very little is understood regarding CNS changes that lead to various cognitive impairments among people with impaired glucose tolerance. Even less is
understood regarding the differences between high performing diabetic patients versus the low performing diabetics. The purpose of this study was to
examine the neurochemical profile differences between low capacity runner rats (LCR) and high capacity runner rats (HCR) using proton magnetic
resonance spectroscopy at 7.0 Tesla. Findings suggest that LCR rats have elevated taurine, myo-inositol, glutamate and choline containing compounds
compared to HCR rats consistent with similar findings in diabetic patients.
2382. Effect of Lactate on FMRI Responses Under Hypoglycemia
Lihong Jiang 1 , Basavaraju G. Sanganahalli 1 , Peter Herman 1 , Raimund Herzog 1 , Robert Sherwin 1 ,
Fahmeed Hyder 1 , Douglas Rothman 1 , Kevin Behar 1
1 Yale University, New Haven, CT, United States
We investigated BOLD responses to forepaw stimulation under insulin-induced acute hypoglycemic condition, as well as effect of lactate infusion under
hypoglycemia condition. All high field fMRI experiments were conducted in α-chloralose anesthetized rats. The magnitude of the BOLD response in
primary somatosensory (S1 FL ) region decreased from euglycemic to hypoglycemic conditions. Upon lactate infusion, under hypoglycemic condition,
transiently increased S1 activities, but also recruited regions beyond S1 FL . These results will benefit the understanding of brain function and metabolism, as
well as the role of alternative fuels under hypoglycemic condition.
MRS of Animal Brain
Hall B Tuesday 13:30-15:30
2383. Simultaneous Detection of Metabolism of Different Substrates in the Carboxylic/amide Region Using in
Vivo 13 C MRS
Yun Xiang 1 , Jun Shen 1
1 National Institute of Mental Health, Bethesda, MD, United States
In the carboxylic/amide region, brain 13 C signals can only have one one-bond 13 C- 13 C homonuclear coupling. As such only doublets (with a 13 C- 13 C coupling
of ~50 Hz) and singlets exist in this region. The large one-bond 13 C- 13 C J coupling and the lack of interference from other isotopomers provide a unique