TRADITIONAL POSTER - ismrm

Poster Sessions
condition for simultaneous detection of metabolism of different substrates. Examples of co-infusion of [ 13 C 6 ]-D-glucose and [1- 13 C] acetate as well as coinfusion
of [ 13 C 6 ]-D-glucose and [1,3- 13 C 2 ] hydroxybutyrate are shown to demonstrate in vivo simultaneous detection of different metabolic pathways in the
brain using 13 C MRS of the carboxylic/amide region.
2384. Alcohol as a Substitute for Acetate in 13 C MRS Study of Brain Metabolism
Yun Xiang 1 , Jun Shen 1
1 National Institute of Mental Health, Bethesda, MD, United States
Acetate is a glia-specific subtract and has been used to study brain metabolism. Potential risk in intravenously infusing sodium acetate to patients is unknown
in many disorders. The effect of alcohol (ethanol) consumption is well understood. Alcohol is predominantly metabolized into acetate in the liver. In the
present study, steady sate 13 C spectra of rat brain acquired after administration of [1- 13 C] ethanol were found to be highly similar to spectra obtained using
[1- 13 C] acetate, suggesting that oral administration of [1- 13 C] alcohol could replace intravenous infusion of sodium [1- 13 C] acetate in certain studies when the
direct effect of alcohol is unimportant for the subject of the study.
2385. Neurochemical Profile of the Rat Lateral Septum Investigated with 1H-MRS
Nathalie Just 1,2 , Maria-Isabel Cordero Campana 3,4 , Guillaume Poirier 3 , Hongxia Lei 1,2 , Carmen Sandi 3 ,
Rolf Gruetter 1,5
1 LIFMET, CIBM, EPFL, Lausanne, Switzerland; 2 Department of Radiology, UNIL, Lausanne, Switzerland; 3 Laboratory of
Behavioural Genetics, EPFL, Brain and Mind Institute, Lausanne, Switzerland; 4 Department of Child and Adolescent Psychiatry,
HUG, Geneve, Switzerland; 5 Department of Radiology, UNIL and HUG, Lausanne and Geneva, Switzerland
The rat lateral septum has been shown to be involved in the expression of anxiety-behaviors such as those involved in conflict procedures. The
neurochemical profile of the lateral septum has however never been characterized using proton magnetic resonance spectroscopy in the rat. In the present
work, the neurochemical profile of the rat lateral septum was measured at 9.4T using 1H-MRS demonstrating significant changes compared to similar data
measured in unspecific rat brain regions. It appears essential to characterize the metabolic profile of specific brain areas with accuracy using 1H MRS.
2386. Lesions of Ventral Tegmental Area in the Mouse and Consequences on Glutamate, Gaba and
Glutamine Levels Assessed Using Proton 1H Mrs.
Carine Chassain 1,2 , Guy Bielicki 1 , Yildiz Zengin 2 , Jean-Pierre Renou 1 , Franck Durif, 2,3
1 NMR plateform, INRA, Saint Genes Champanelle, France; 2 EA 3845, University of Auvergne, Clermont-Ferrand, France; 3 service
Neurology, CHU Clermont-Ferrand, Clermont-Ferrand, France
Parkisnon's disease, 1H MRS, Glutamte, Glutamine, GABA, nucleus accumbens
2387. NMR Investigations of Excitatory and Inhibitory Neurotransmission in Mouse Brain
Anant Bahadur Patel 1 , Vivek Tiwari 1 , A.L. Susmitha 1 , K.S. Varadarajan 1
1 NMR Microimaging and Spectroscopy, Centre for Cellular and Molecular Biology, Hyderabad, Andhra Pradesh, India
Knowledge of neurotransmitter metabolism is very important for understanding the pathophysiology of neurological disorders. In the present study we have
investigated neuronal TCA cycle and neurotransmitter cycle flux in different brain regions of C57BL6 mouse. Mice were infused with [1,6- 13 C 2 ]glucose for
different time ranging from 7 to 90 min or [2- 13 C]acetate for ~90 min. Brain metabolite levels and 13 C labeling of amino acids were measured with 1 H-[ 13 C]-
NMR spectroscopy at 14T NMR spectrometer. The metabolite levels were distinct in different regions of the brain. Glutamatergic rate was higher in cortex
while GABAergic was more in cerebellum and olfactory bulb.
2388. Regional Absolute Quantification in Neurochemical Profile of the Canine Brain: Investigation by
Proton Nuclear Magnetic Resonance Spectroscopy and Tissue Extraction
Dong-Cheol Woo 1 , Chi-Bong Choi 2 , Sung-Ho Lee 3 , Eunjung Bang 4 , Sang-Soo Kim 1 , Hyang-Shuk Rhim 1 ,
Sang-Young Kim 1 , Bo-Young Choe 1
1 The Catholic University of Korea, Seoul, Korea, Republic of; 2 Kyung-Hee University of Korea; 3 Konkuk university of Korea; 4 Korea
Basic Science Institute
This study was to characterize the regional neurochemical profiles of canine brain using NMRS, tissue extraction, and external simulated phantom
concentration quantification. The occipital, frontal, and temporal lobes, thalami, cerebellar cortices, and spinal cords of adult beagles were obtained, and
NMR samples were prepared using M/C extraction method. The metabolite concentrations in canine brain tissues were measured and compared with those
found in human and rat brain. In addition, the cross peaks of brain metabolites were identified using 2D-COSY. This study demonstrated the absolute
quantification of canine neuronal parts using MRS, with tissue extraction used to measure metabolite concentrations.
2389. In Vivo Evidence for Ketamine-Induced Neurochemical Changes in Rat Prefrontal Cortex: An Animal
Model of Schizophrenia
Sang-Young Kim 1 , Hyun-Sung Lee 2 , Eunjung Bang 2 , Hyun-Ju Kim 2 , Sung-Ho Lee 3 , Do-Wan Lee 1 , Dong-
Cheol Woo 1 , Chi-Bong Choi 4 , Bo-Young Choe 1
1 Department of Biomedical Engineering, The Catholic University of Korea, Seoul, Korea, Republic of; 2 Korea Basic Science Institute,
Korea, Republic of; 3 Department of Veterinary Surgery, Konkuk University, Seoul, Korea, Republic of; 4 Department of Radiology,
Kyunghee University Medical Center, Seoul, Korea, Republic of
The ketamine, a NMDA receptor antagonist, impair prefrontal cortex (PFC) function in the rat and produce symptoms similar to schizophrenia. In this study,
we used in vivo and in vitro 1H-NMR spectroscopy to examine the brain metabolism of rat treated with subanesthetic dose of ketamine. In vivo data for
Glu/Gln abnormalities in ketamine-treated rats may support the hypotheses of glutamate dysfunction for schizophrenia. In addition lower metabolic level of
NAA in rats treated with ketamine may indicate reduced neuronal viability. Therefore our findings suggest that the neurochemical alterations induced by
ketamine may provide the foundation for pathophysiological models of schizophrenia.