TRADITIONAL POSTER - ismrm

Poster Sessions
997.
19 F Magnetic Resonance Quantification of SiRNA Delivery Via Perfluorocarbon Nanoparticle
Emulsions
Jacob Wheatley Myerson 1 , Megan M. Kaneda 1 , Gregory M. Lanza 1,2 , Samuel A. Wickline 1,2
1 Biomedical Engineering, Washington University, Saint Louis, MO, United States; 2 Department of Medicine, Washington University,
Saint Louis, MO, United States
Perfluorocarbon nanoparticles were functionalized to deliver VCAM-1 siRNA to human melanoma cells. Delivery was quantified via 19 F magnetic
resonance spectroscopy. For future in vivo studies, an MR-trackable delivery agent would aid in the determination of localization of siRNA delivery to
specific tissues.
998. The Loss of Sodium Homeostasis and Apoptosis During Rodent Glioma Chemotherapy
Victor D. Schepkin 1 , Cathy W. Levenson 2 , Fabian F. Calixto-Bejarano 2 , William W. Brey 1 , Petr L. Gor'kov 1
1 CIMAR, NHMFL/FSU, Tallahassee, FL, United States; 2 College of Medicine, FSU, Tallahassee, FL, United States
The goal of the present study was to assess the hypothesis that an in vivo increase of intracellular sodium is one of the first and a crucial stage during cancer
therapy. Experiments were performed using high resolution sodium and diffusion MRI at 21.1T and rodent glioma model. During efficient BCNU
chemotherapy, tumor sodium reaches a plateau indicating a complete loss of Na homeostasis at day 4 following the initiation of therapy. Dose dependent
responses of intracellular sodium can serve as a very early biomarker for the onset of apoptosis and forecast tumor elimination.
999. In Vivo Multicolor Imaging of Perfluorocarbon Emulsions Using Ultrafast Spectroscopic Imaging (F-
UTSI)
Muhammed Yildirim 1,2 , Shelton D. Caruthers 3 , Aart J. Nederveen 4 , Jaap Stoker 4 , Rolf Lamerichs 1
1 Philips Research, Eindhoven, Netherlands; 2 Biomedical NMR, Department of Biomedical Engineering, Eindhoven University of
Technology, Eindhoven, Netherlands; 3 Washington University, St Louis, MO, United States; 4 Department of Radiology, Academic
Medical Center, Amsterdam, Netherlands
In vivo 3D F-uTSI, can distinguish various 19F compounds based on chemical shift differences allowing for ‘multicolor’ imaging, without additional
increase in scan time. 3D F-uTSI is an efficient spectral imaging technique; data with a resolution of 48x48x14 can be recorded with a scan-time of 6:23m.
The spectra of several Perfluorocarbon emulsion can be uniquely identified from these data. Main advantage of the spectroscopic, multicolor, imaging
approach is that the spectral images of the different PFC agents are recorded at the same time and, furthermore, complex biological process can be imaged
using co-injection of differently functionalized PFC emulsions.
1000. Sodium MR Imaging in the Study of Hepatic Encephalopathy
Nadim Jon Shah 1,2 , Vincent Gras 1 , Anna-Maria Oros-Peusquens 1 , Eberhard Daniel Pracht 1 , Sandro
Romanzetti 1 , Gerald Kircheis 3 , Dieter Häussinger 3
1 Institute of Neuroscience and Medicine 4, Medical Imaging Physics, Forschungszentrum Jülich GmbH, Jülich, Germany; 2 Faculty of
Medicine, Department of Neurology, RWTH Aachen University, Aachen, Germany; 3 Klinik f. Gastroenterologie, Hepatologie und
Infektiologie, Universitätsklinikum Düsseldorf, Düsseldorf, Germany
Sodium MRI provides an new tool for the analysis of mechanisms involved in hepatic encephalopathy (HE), a neuropsychiatric complication of liver failure.
Hyperintense regions observed in the basal ganglia in proton images of HE patients are expected to show changes in sodium images due to the involvement
of hyponatraemia in HE. Using sodium MRI, expected subtle changes are investigated using the structural information - delineation of structures of interest -
provided by anatomical MR images. Based on the analysis of a large group of patients versus controls, this research potentially provides novel, valuable
information concerning metabolic changes in HE.
1001. Efficient B0-Inhomogeneity Insensitive TQF 23 Na Imaging.
Lazar Fleysher 1 , Niels Oesingmann 2 , Matilde Inglese 3
1 Department of Radiology, NYU School of Medicine, New York, United States; 2 Siemens Medical Solutions USA, Inc., United
States; 3 Departments of Radiology and Neurology, NYU School of Medicine, New York, United States
We present a new 12-step triple-quantum filtering phase-cycling scheme based on three RF pulses which allows compensation of B 0 variations both with and
without ancillary B 0 -map information. The method offers 40% higher SNR efficiency compared to the previously developed phase-cycling schemes.
1002. Sodium MRI Multi-Echo Sequence for Simultaneous Ultra-Short Echo Imaging and T2L* Mapping at
7T with a 12 Channel Phased-Array Coil
David Thomas Pilkinton 1 , Mark A. Elliott 1 , Jeremy Magland 2 , Ravinder Reddy 1
1 Center for Magnetic Resonance and Optical Imaging, University of Pennsylvania, Philadelphia, PA, United States; 2 Department of
Radiology, University of Pennsylvania, Philadelphia, PA
To maximize signal-to-noise ratio (SNR) and minimize relaxation weighting, ultra-short echo (UTE) readouts have been generally been favored for sodium
MRI. UTE methods have also been used to measure total sodium concentration (TSC) in tissues, although this typically requires accurate mapping of the B1
field which is typically noisy and error prone. A more reliable quantitative parameter for sodium MRI is the measurement of the long component of the
transverse relaxation time, T2L*. In this study, we have implemented a robust sodium imaging sequence for simultaneous UTE imaging and T2L*
measurements.