TRADITIONAL POSTER - ismrm

Poster Sessions
1325. Exploration of Mitochondrial Respiration in Isolated Hearts: An Observation from Metabolically
Produced H 2 17 O Using 17 O NMR Spectroscopy
Ming Lu 1,2 , Jessica Spires 1 , Gheorghe D. Mateescu 2,3 , Chris Flask 2,4 , Xin Yu 1,2
1 Biomedical Engineering, Case Western Reserve University, Cleveland, OH, United States; 2 Case Center for Imaging Research, Case
Western Reserve University, Cleveland, OH, United States; 3 Chemistry, Case Western Reserve University; 4 Radiology, Case Western
Reserve University, Cleveland, OH, United States
Cardiac applications of 17 O NMR for evaluating mitochondrial function have been limited due to the challenge of detecting metabolic H 2 17 O in the vast
background of natural abundance H 2 17 O. In this study, we developed a direct 17 O MR Spectroscopy (MRS) approach to examine the feasibility and
sensitivity of detecting metabolically produced H 2 17 O in isolated rat hearts perfused with 17 O 2 -enriched Krebs buffer. Mitochondrial production of H 2 17 O was
monitored by dynamic 17 O spectroscopy. Oxygen consumption rate (MVO 2 ) was determined by least-square fitting of a compartmental model to NMR data.
An increase of MVO 2 was observed under elevated workload induced by high Ca 2+ concentration.
1326. In Vivo T1 Mapping of 31 P Metabolites at Short TR
Mahon L. Maguire 1 , Hannah Barnes 1 , Stefan Neubauer 1 , Jurgen E. Schneider 1
1 Dept Cardiovascular Medicine, University of Oxford, Oxford, Oxon, United Kingdom
Quantitation of the energetics of the heart requires accurate tissue specific T1 values for the 31 P metabolites involved. Acquisition of 31 P spectroscopic data
at high resolution necessitates the use of a short TR relative to the T1 of the metabolites. High resolution 31 P chemical shift imaging was employed to take
spatially resolved 2, 3 and 4 flip angle T1 measurements. Phantom studies are presented investigating the optimal parameters for T1 mapping in vivo. A
dual angle T1 measurement employing flip angles of 30 and 15° is optimal where TR/T1